Abstract This study reports the synthesis and characterization of PbMoO4 nanocrystals and the antibacterial properties and modulation of their antibiotic activity. Lead molybdate nanocrystals were obtained through the conventional hydrothermal method, and the structural, vibrational and morphological properties of the sample were determined using X-ray diffraction, Raman and infrared spectroscopy, and scanning electron microscopy images. The obtained samples present scheelite-type tetragonal structure (space group I41/a) without secondary phases, and the Raman spectrum shows the formation of PbMoO4 structurally ordered at short-range, confirming the quality of the samples. The antibacterial and antibiotic-modulation activities were performed in triplicate using the microdilution method. The minimum inhibitory concentration (MIC) of antibiotics alone or in association with PbMoO4, were demonstrated using geometric mean. When assayed directly, the PbMoO4 presented a MIC ≥ 1024 μg/mL against all bacterial strains tested. When in association with the gentamicin against Staphylococcus aureus, a synergism was observed reducing 37.5% the MIC. However, in the assays against the Gram-negative strains was observed that the presence of lead affected the effect of the antibiotic, leading to an antagonism.

中文翻译：

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PbMoO 4 纳米晶的合成、表征和抗菌性能

摘要 本研究报告了 PbMoO4 纳米晶体的合成和表征及其抗菌性能和抗菌活性的调节。通过常规水热法获得钼酸铅纳米晶，并利用X射线衍射、拉曼光谱和红外光谱以及扫描电子显微镜图像确定样品的结构、振动和形态特性。所得样品呈白钨型四方晶结构（空间群I41/a），无二次相，拉曼光谱显示形成了短程结构有序的PbMoO4，证实了样品的质量。使用微量稀释方法一式三份进行抗菌和抗生素调节活性。使用几何平均值证明单独使用抗生素或与 PbMoO4 联合使用的最低抑菌浓度 (MIC)。当直接检测时，PbMoO4 对所有测试的细菌菌株的 MIC ≥ 1024 μg/mL。当与庆大霉素联合对抗金黄色葡萄球菌时，观察到协同作用降低 37.5% 的 MIC。然而，在针对革兰氏阴性菌株的测定中观察到铅的存在影响抗生素的作用，导致拮抗作用。