Aiming at improving reliability and tedious analysis time in conventional cell membrane chromatography, an improved bioaffinity sample pretreatment technique with enhanced stability was developed to fast screen and extract potential allergenic components from traditional Chinese medicine injections. In this study, rat basophilic leukemia-2H3 cell membrane coated silica particles (RBL-2H3/CMCSPs) were fabricated by irreversible adsorption between the cell membrane and silica and self-fusion of the cell membrane, which could simulate drug–receptor interactions in vitro. Also, benefiting from the use of paraformaldehyde, the average recoveries of the six batches of RBL/CMCSPs were 90.2% with a relative standard deviation of less than 7.8%, showing that the stability of the materials was remarkably improved compared to materials without fixation. After the successful characterization by spectroscopic and imaging instruments, the prepared RBL-2H3/CMCSPs exhibited desirable adsorption capacity and selectivity. The RBL-2H3/CMCSPs combined with high performance liquid chromatography coupled with time of flight mass spectrometry were then successfully applied to screen and identify two potential allergenic components from huangqi injection, the allergenic activities of which were further investigated by β-hexosaminidase release assay and histamine release assay in vitro. Overall, this work provides a good platform for the fabrication of bioaffinity sample pretreatment materials with high stability and a long lifespan, which can be a time-saving and energy-saving bioaffinity method to rapidly screen and preconcentrate target compounds from complex samples.

中文翻译：

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改进的细胞膜生物亲和样品预处理技术，具有增强的稳定性，可从中药注射剂中筛选潜在的致敏成分

为了提高传统细胞膜色谱的可靠性和繁琐的分析时间，开发了一种具有更高稳定性的改进的生物亲和样品预处理技术，可以从中药注射剂中快速筛选和提取潜在的致敏成分。在这项研究中，大鼠嗜碱性粒细胞白血病2H3细胞膜包被的二氧化硅颗粒（RBL-2H3 / CMCSPs）是通过细胞膜和二氧化硅之间不可逆的吸附以及细胞膜的自融合来制备的，这可以模拟体外的药物-受体相互作用。此外，得益于多聚甲醛的使用，六批RBL / CMCSP的平均回收率为90.2％，相对标准偏差小于7.8％，表明与未固定的材料相比，该材料的稳定性得到了显着改善。在通过光谱和成像仪器成功表征后，制备的RBL-2H3 / CMCSPs表现出理想的吸附能力和选择性。然后成功地将RBL-2H3 / CMCSPs与高效液相色谱结合飞行时间质谱联用，从黄芪注射液中筛选和鉴定了两种潜在的致敏成分，并通过β-己糖胺酶释放试验进一步研究了其致敏活性。组胺释放测定体外。 总的来说，这项工作为制备具有高稳定性和长寿命的生物亲和样品预处理材料提供了一个良好的平台，这是一种省时又节能的生物亲和方法，可以从复杂样品中快速筛选和预浓缩目标化合物。