Microbiological Research ( IF 6.7 ) Pub Date : 2017-11-11 , DOI: 10.1016/j.micres.2017.11.005 Donatien Kaboré , Mérilie Gagnon , Denis Roy , Hagrétou Sawadogo-Lingani , Bréhima Diawara , Gisèle LaPointe
Forty Bacillus isolates obtained from maari (used as condiment in Burkina Faso) including 17 B. subtilis, 4 B. circulans, 7 B. pumilus and 6 B. licheniformis were investigated for use as starter cultures in maari production. The isolates were screened by PCR for the sfp gene responsible for the production of the lipopeptide biosurfactant, surfactin. The sfp gene was detected in all of the seventeen B. subtilis isolates, in 2 out of 7 B. pumilus, in 4 out of 6 B. licheniformis whereas no B. circulans was positive for the sfp gene by PCR screening. Furthermore, all the 40 Bacillus spp. were screened for biosurfactant production and inhibitory activity against Aspergillus flavus, A. niger, A. versicolor and Rhizopus oryzae. Results demonstrated a relationship between the presence of the sfp gene and the antifungal activity and biosurfactant production of Bacillus isolates. In addition, molecular typing of the 17 B. subtilis isolates by Multilocus Sequence Typing (MLST) resulted in 15 Sequence Types, one of them included three strains. Randomly Amplified Polymorphic DNA-PCR (RAPD-PCR), used for B. licheniformis, B. megaterium, B. circulans and B. pumilus revealed that the inhibitory activity and biosurfactant production were strain-dependent. Finally, the detection of chitinase (chi) and β-glucanase (glu) biosynthesis genes was found to be associated with the antifungal activity for 16 B. subtilis isolates. The present work provides a greater understanding of the antifungal activity and biosurfactant production ability within the Bacillus spp. isolated from maari and contributes to the selection of Bacillus isolates to be used as starter cultures for controlled production of maari.
中文翻译:
基于抗真菌特性快速筛选马雷氏发酵剂培养物
研究了从maari(在布基纳法索用作调味品)获得的40种芽孢杆菌分离株,包括17 B. subtilis, 4 B. circulans,7 B. pumilus和6 B. licheniformis,用作maari生产中的发酵剂。通过PCR筛选分离物以寻找负责产生脂肽生物表面活性剂表面活性剂的sfp基因。在SFP中的所有17个的检测基因的枯草芽孢杆菌菌株,在2选自7的短小芽孢杆菌,在出6 4的地衣芽孢杆菌,而没有环状芽孢杆菌PCR筛选对sfp基因呈阳性。此外,所有40个芽孢杆菌属。筛选生物表面活性剂生产和对抑制活性黄曲霉,黑曲霉,杂色曲霉和米根霉。结果证明了sfp基因的存在与芽孢杆菌分离株的抗真菌活性和生物表面活性剂产生之间的关系。另外,通过多基因座序列分型(MLST)对17株枯草芽孢杆菌分离株进行分子分型产生了15种序列类型,其中之一包括三个菌株。随机扩增多态性DNA-PCR(RAPD-PCR),用于地衣芽孢杆菌,巨大芽孢杆菌,圆形芽孢杆菌和短小芽孢杆菌显示抑制活性和生物表面活性剂的产生依赖于菌株。最后,发现几丁质酶(chi)和β-葡聚糖酶(glu)生物合成基因的检测与16株枯草芽孢杆菌分离物的抗真菌活性有关。本工作提供了对芽孢杆菌属中的抗真菌活性和生物表面活性剂生产能力的更深入的了解。分离自maari并有助于选择芽孢杆菌分离物,用作受控生产maari的起始培养物。