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Cytogenetics alteration in adult men involved in the recycling of electronic wastes
Environment International ( IF 11.8 ) Pub Date : 2017-12-22 , DOI: 10.1016/j.envint.2017.12.026
Yanan Du , Yan Wang , Liqing Du , Chang Xu , Kaihua Ji , Jinhan Wang , Qiang Liu

In this data article, 146 villagers (exposed group) were randomly selected from the workers who involved in the e-wastes recycling directly as a daily job in Tianjin. Control group, including 121 villagers, came from another town without e-waste disposal sites. Chromosomal aberrations (CA) and cytokinesis blocking micronucleus (CBMN) were performed to detect the cytogenetic effect for each subject. DNA damage was detected using comet assay; the DNA percentage in the comet tail (TDNA%), tail moment (TM), and Olive tail moment (OTM) were recorded to describe DNA damage to lymphocytes and spermatozoa. Routine semen analysis, spermatozoa motility and morphology were analyzed. The RT2Profiler PCR array was used to measure levels of expression of 84 genes related to quality of DNA. It showed significant relationships between CA, CBMN, DNA damage and exposure time in exposure subjects. The alteration of sperm motility rate, abnormality rate and total sperm counts had association with exposure time and age.



中文翻译:

成年男子参与电子废物回收的细胞遗传学改变

在此数据文章中,从天津从事日常电子垃圾回收工作的工人中随机抽取了146名村民(暴露组)。包括121个村民在内的对照组来自另一个没有电子垃圾处理场的镇。进行染色体畸变(CA)和细胞分裂阻滞微核(CBMN),以检测每个受试者的细胞遗传学作用。使用彗星试验检测DNA损伤;记录彗星尾巴(TDNA%),尾矩(TM)和橄榄尾矩(OTM)中的DNA百分比,以描述DNA对淋巴细胞和精子的损伤。常规精液分析,精子活力和形态进行了分析。RT 2Profiler PCR阵列用于测量与DNA质量相关的84个基因的表达水平。结果表明,暴露对象的CA,CBMN,DNA损伤与暴露时间之间存在显着的关系。精子活力,异常率和精子总数的变化与暴露时间和年龄有关。

更新日期:2017-12-23
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