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Cytotoxic, proapoptotic and antioxidative potential of flavonoids isolated from propolis against colon (HCT-116) and breast (MDA-MB-231) cancer cell lines
Food Research International ( IF 8.1 ) Pub Date : 2017-12-22 , DOI: 10.1016/j.foodres.2017.12.056
Nenad L. Vukovic , Ana D. Obradovic , Milena D. Vukic , Danijela Jovanovic , Predrag M. Djurdjevic

Isolated and structurally confirmed, eleven flavonoids from propolis were examined for their cytotoxicity toward human colon cancer and human breast cancer cells. Their effect on induction of apoptosis and their antioxidative activities were also evaluated. Six flavonoids induced cytotoxic effects in both cell lines. Luteolin had a marked effect on both cell lines, especially on HCT-116 cells (IC50 72 h, 66.86 μM). Also, luteolin was observed to have the highest apoptotic potential after 72 h treatment of examined cell lines (27.13% and 37.09%, respectively). Myricetin exhibited selective inhibition of cell growth (IC50 114.75 μM) and induced apoptosis in MDA-MB-231 cells only. Luteolin and galangin exhibited prooxidative properties 24 h after the treatment in HCT-116 cells, while myricetin induced prooxidative effects in MDA-MB-231 cells. On the other hand, selected flavonoids exhibited antioxidative properties 72 h after the treatment, decreasing superoxide anion radical and nitrite levels in both cell lines. Cytotoxic and proapoptotic effects on colon and breast cancer cell lines and the influence on their redox status make tested flavonoids good candidates for developing new anticancer drugs.



中文翻译:

从蜂胶中分离出的黄酮类化合物具有抗结肠癌(HCT-116)和乳腺癌(MDA-MB-231)癌细胞系的细胞毒性,促凋亡和抗氧化能力

分离并在结构上得到确认的蜂胶中的11种类黄酮对人结肠癌和人乳腺癌细胞具有细胞毒性。还评估了它们对诱导细胞凋亡的作用及其抗氧化活性。六种类黄酮在两种细胞系中均诱导了细胞毒性作用。木犀草素对两种细胞系都有显着影响,尤其是对HCT-116细胞(IC 50 72 h,66.86μM)。此外,观察到木犀草素在处理所检查的细胞系72小时后具有最高的凋亡潜能(分别为27.13%和37.09%)。杨梅素表现出对细胞生长的选择性抑制(IC 50114.75μM),仅在MDA-MB-231细胞中诱导凋亡。木犀草素和高良姜精在HCT-116细胞中处理后24 h表现出促氧化作用,而杨梅素在MDA-MB-231细胞中诱导促氧化作用。另一方面,选定的类黄酮在处理72小时后表现出抗氧化特性,从而降低了两种细胞系中的超氧阴离子自由基和亚硝酸盐水平。对结肠和乳腺癌细胞系的细胞毒性和促凋亡作用以及对它们的氧化还原状态的影响使经测试的类黄酮成为开发新的抗癌药物的良好候选者。

更新日期:2017-12-22
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