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Size-Dependent Inertial Focusing Position Shift and Particle Separations in Triangular Microchannels
Analytical Chemistry ( IF 7.4 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1021/acs.analchem.7b03851
Jeong-ah Kim 1 , Je-Ryung Lee 2 , Tae-Jin Je 2 , Eun-chae Jeon 2 , Wonhee Lee 1, 3
Affiliation  

A recent study of inertial microfluidics within nonrectangular cross-section channels showed that the inertial focusing positions changes with cross-sectional shapes; therefore, the cross-sectional shape can be a useful control parameter for microfluidic particle manipulations. Here, we conducted detail investigation on unique focusing position shift phenomena, which occurs strongly in channels with the cross-sectional shape of the isosceles right triangle. The top focusing positions shift along the channel walls to the direction away from the apex with increasing Reynolds number and decreasing particle size. A larger particle with its center further away from the side walls experiences shear gradient lift toward the apex, which leads to an opposite result with changes of Reynolds and particle size. The focusing position shift and the subsequent stabilization of corner focusing lead to changes in the number of focusing positions, which enables a novel method for microparticle separations with high efficiency (>95%) and resolution (<2 μm). The separation method based on equilibrium focusing; therefore, the operation is simple and no complex separation optimization is needed. Moreover, the separation threshold can be easily modulated with flow rate adjustment. Rare cell separation from blood cell was successfully demonstrated with spiked MCF-7 cells in blood by achieving the yield of ∼95% and the throughput of ∼106 cells/min.

中文翻译:

三角形微通道中尺寸相关的惯性聚焦位置偏移和粒子分离

对非矩形横截面通道内的惯性微流体的最新研究表明,惯性聚焦位置随横截面形状而变化;因此,横截面形状可以作为微流体颗粒处理的有用控制参数。在这里,我们对独特的对焦位置偏移进行了详细调查现象在等腰直角三角形横截面的通道中强烈发生。随着雷诺数的增加和粒径的减小,顶部聚焦位置沿着通道壁向远离顶点的方向移动。中心距侧壁较远的较大粒子经历了朝向顶点的剪切梯度提升,这导致了雷诺数和粒径变化的相反结果。聚焦位置移动和随后的转角聚焦稳定化导致聚焦位置数量的变化,从而实现了高效(> 95%)和分离度(<2μm)的新型微粒分离方法。基于平衡聚焦的分离方法;因此,操作简单,不需要复杂的分离优化。而且,分离阈值可通过流速调节轻松调节。掺入血液的MCF-7细胞成功地证明了从血细胞中稀少的细胞分离,达到了约95%的产率和约10的通量6格/分钟
更新日期:2018-01-10
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