An aldehyde functionalized ionic liquid, (3-(3-formyl-4-hydroxybenzyl)-3-methylimidazolium hexafluorophosphate) (CHO-IL) has been employed herein as a multiple host platform for the covalent immobilization of mediator as well as enzyme. The CHO-IL was immobilized on electrochemically reduced graphene oxide (ERGO) through the π-π stacking of hydroxybenzyl and imidazolium groups with ERGO and subjected to further covalent attachment of Azure A (Azu-A) mediator or glucose oxidase (GOx) enzyme. Electroactive, water soluble organic dye Azu-A was effectively immobilized to the host IL through simple Schiff base reaction. Azu-A was rendered leak-free in the electrode setup and also responded well for the amperometric determination of H₂O₂ over a linear range of 0.03–1 mM with a detection limit and sensitivity of 11.5 µM and 133.2 µA mM⁻¹ cm⁻², respectively. Further, attempts were made to explore the CHO-IL platform for the covalent immobilization of GOx enzyme which served well in retaining the enzyme nativity, reactivity and stability. Under optimized conditions, mediatorless GOx biosensor developed based on direct electrochemistry has exhibited an impressive analytical signal towards glucose detection in the linear range of 0.05–2.4 mM with a detection limit and sensitivity of 17 µM and 17.7 µA mM⁻¹ cm⁻², respectively. The reliability of the proposed Azu-A based chemical sensor and GOx based biosensor towards the determination of H₂O₂ and glucose in the real samples have been demonstrated. The remarkable analytical parameters and long term stability of both the sensors could be envisioned as a result of facile immobilization platform and immobilization strategy.

醛官能化的离子液体（3-（3-甲酰基-4-羟基苄基）-3-甲基咪唑六氟磷酸盐）（CHO-IL）在本文中已用作共价固定介体和酶的多宿主平台。CHO-IL通过羟基苄基和咪唑鎓基团与ERGO的π-π堆叠固定在电化学还原的氧化石墨烯（ERGO）上，并进一步与Azure A（Azu-A）介体或葡萄糖氧化酶（GOx）酶共价连接。通过简单的席夫碱反应，将电活性水溶性有机染料Azu-A有效地固定在宿主IL上。使Azu-A在电极设置中无泄漏，并且对于H ₂ O ₂的安培测定也反应良好线性范围为0.03-1 mM，检测极限和灵敏度分别为11.5 µM和133.2 µA mM ^-1  cm ^-2。此外，尝试探索用于共价固定GOx酶的CHO-IL平台，该平台很好地保持了酶的天然性，反应性和稳定性。在最佳条件下，基于直接电化学技术开发的无介体GOx生物传感器在线性检测范围为0.05–2.4 mM的葡萄糖中显示出令人印象深刻的分析信号，其检测极限和灵敏度分别为17 µM和17.7 µA mM ^-1  cm ^-2。 。拟议的基于Azu-A的化学传感器和基于GOx的生物传感器对H ₂ O测定的可靠性₂和真实样品中的葡萄糖已被证明。简便的固定平台和固定策略可预见两个传感器均具有出色的分析参数和长期稳定性。