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Multi-channel biphasic calcium phosphate granules as cell carrier capable of supporting osteogenic priming of mesenchymal stem cells
Materials & Design ( IF 8.4 ) Pub Date : 2018-03-01 , DOI: 10.1016/j.matdes.2017.12.040
Celine D.G. Abueva , Chan Mi Park , Boram Kim , Byong-Taek Lee

Abstract Advances in bone tissue engineering include versatile and intricate biomaterial scaffolds in combination with stem cells for enhanced bone regeneration. In this study, a unique scaffold with multi-channels designed to allow cell infiltration within its pores was investigated for its capability to serve as a stable platform for adhesion and osteogenic priming of mesenchymal stem cells. The biphasic calcium phosphate multi-channel granule consisted of 60% hydroxyapatite and 40% β-tricalcium phosphate. Successful loading and retention of isolated and expanded rat bone marrow-derived mesenchymal stem cells (rBMSCs) were observed. The cells proliferated within the micro-channels starting from the surface then into the channels. The multi-channel granules were also able to support osteogenic priming of rBMSCs in 2D culture without the aid of a growth factor. Alkaline phosphatase, type I collagen, and runt-related transcription factor 2 expressions were detected with high osteopontin marker expression in as early as 7 days, which persisted for 14 days of culture under osteogenic condition. Results confirmed commitment towards osteogenic lineage of rBMSCs that have attached and grown onto the surface of the multi-channel granules and thus have high potential as a cell-scaffold based approach in bone regenerative medicine.

中文翻译:

多通道双相磷酸钙颗粒作为能够支持间充质干细胞成骨启动的细胞载体

摘要 骨组织工程的进展包括多功能和复杂的生物材料支架与干细胞相结合以增强骨再生。在这项研究中,研究了一种具有多通道的独特支架,其设计允许细胞在其孔内浸润,因为它具有作为间充质干细胞粘附和成骨启动的稳定平台的能力。双相磷酸钙多通道颗粒由 60% 羟基磷灰石和 40% β-磷酸三钙组成。观察到分离和扩增的大鼠骨髓间充质干细胞 (rBMSCs) 的成功加载和保留。细胞在微通道内从表面开始增殖,然后进入通道。多通道颗粒还能够在没有生长因子帮助的情况下支持 rBMSCs 在 2D 培养中的成骨启动。早在 7 天就检测到碱性磷酸酶、I 型胶原蛋白和矮小相关转录因子 2 表达,并具有高骨桥蛋白标记表达,并在成骨条件下持续培养 14 天。结果证实了对 rBMSCs 的成骨谱系的承诺,这些细胞已经附着并生长到多通道颗粒的表面,因此在骨再生医学中作为基于细胞支架的方法具有很高的潜力。
更新日期:2018-03-01
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