当前位置: X-MOL 学术Microbiol. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative transcriptomic analysis of Cerrena unicolor revealed differential expression of genes engaged in degradation of various kinds of wood
Microbiological Research ( IF 6.7 ) Pub Date : 2017-12-20 , DOI: 10.1016/j.micres.2017.12.007
Grzegorz Janusz , Andrzej Mazur , Jerzy Wielbo , Piotr Koper , Kamil Żebracki , Anna Pawlik , Beata Ciołek , Andrzej Paszczyński , Agnieszka Kubik-Komar

To explore the number of enzymes engaged by Cerrena unicolor FCL139 for wood degradation, the transcriptomes of the fungus growing on birch, ash, maple sawdust and the control liquid medium were analyzed. Among 12,966 gene models predicted for the C. unicolor genome, 10,396 all-unigenes were detected, of which 9567 were found to be expressed in each of the tested growth media. The highest number (107) of unique transcripts was detected during fungus growth in the control liquid medium, while the lowest number (11) – in the fungal culture comprising maple saw dust. Analysis of C. unicolor transcriptomes identified numerous genes whose expression differed substantially between the mycelia growing in control medium and each of the sawdust media used, with the highest number (828) of upregulated transcripts observed during the fungus growth on the ash medium. Among the 294 genes that were potentially engaged in wood degradation, the expression of 59 was significantly (p < .01) changed in the tested conditions. The transcripts of 37 of those genes were at least four times more abundant in the cells grown in all sawdust media when compared to the control medium. Upregulated genes coding for cellulases and, to a lower extent, hemicellulases predominated during fungus growth on sawdust. Transcripts encoding cellulolytic enzymes were the most abundant in mycelia grown on birch and maple while lower number of such transcripts was detected in fungus growing on ash. The expression pattern of lignolytic activities-coding genes was strongly dependent on the type of sawdust applied for fungus growth medium.



中文翻译:

Cerrena unicolor的比较转录组学分析揭示了参与降解各种木材的基因的差异表达

为了探索Cerrena unicolor FCL139参与木材降解的酶的数量,分析了在桦木,灰,枫木屑和对照液体培养基上生长的真菌的转录组。在预测到的C. unicolor基因组的12,966个基因模型中,检测到10,396个全单基因,其中发现9567个在每种测试的生长培养基中表达。在对照液体培养基中的真菌生长过程中,检测到最大数量的独特转录本(107),而在包含枫木锯末的真菌培养物中检测到的最低数量(11)。单色梭菌的分析转录组鉴定出许多基因,其表达在对照培养基中生长的菌丝体和所用的每种木屑培养基之间存在显着差异,其中在灰烬培养基上的真菌生长过程中观察到最高数量的上调转录本(828)。在可能导致木材降解的294个基因中,有59个的表达在测试条件下发生了显着变化(p <.01)。与对照培养基相比,在所有木屑培养基中生长的细胞中,这些基因中的37个基因的转录物至少富集了四倍。在木屑上的真菌生长过程中,上调编码纤维素酶的基因,并在较低程度上编码半纤维素酶。编码纤维素分解酶的转录本在桦木和枫树上生长的菌丝体中含量最高,而在灰烬上生长的真菌中检出的转录本数量较少。木质素分解活性编码基因的表达模式在很大程度上取决于应用于真菌生长培养基的木屑类型。

更新日期:2017-12-20
down
wechat
bug