To examine novel functions of milk basic protein (MBP) in T-cell-related inflammatory diseases, such as autoimmune diseases and allergies, we evaluated the effects of MBP on the causative responses of ovalbumin (OVA)-specific T cells in a food-allergic enteropathy model, OVA23–3 mice, which express an OVA-specific T-cell receptor gene. The OVA-specific CD4⁺ T cells of the mesenteric lymph nodes (MLN) from OVA23–3 mice were cultured with CD11c⁺ dendritic cells of MLN from BALB/cA mice in the absence or presence of MBP following stimulation with OVA; then the levels of CD69 expression and the levels of cytokine production by CD4⁺ T cells were measured to evaluate activation. The effects of MBP supplementation of OVA 23–3 mice were assessed by feeding a diet containing OVA (OVA diet) with or without MBP for 28 d. Intestinal inflammation, together with activation and cytokine production of CD4⁺ T cells by MLN, as well as femoral bone mineral density, were measured. In in vitro culture, MBP inhibited excess activation and IL-4 production by CD4⁺ T cells. The supplementation of MBP to the OVA diet attenuated OVA-specific IgE production in OVA-diet-fed OVA23–3 mice and slightly resolved developing enteropathy caused by excess IL-4 production by CD4⁺ T cells. Feeding OVA diet to OVA23–3 mice exhibited bone loss accompanied with enteropathy, whereas MBP supplementation prevented bone loss and increased osteoprotegerin, an osteoclastogenesis inhibitory factor, in the mice. The inhibition of T-cell-activation in both MLN and bone marrow by MBP supplementation may help prevent increased IgE levels caused by excessive IL-4 production and bone loss accompanied by enteropathy. Our findings show that MBP may help attenuate both T-cell-related inflammation and bone loss.

为了检查牛奶碱性蛋白（MBP）在T细胞相关炎性疾病（例如自身免疫性疾病和过敏）中的新功能，我们评估了MBP对卵白蛋白（OVA）特异性T细胞在食物中的致病反应的影响。过敏性肠病模型，OVA23–3小鼠，表达OVA特异性T细胞受体基因。在OVA刺激后，在不存在或存在MBP的情况下，将来自OVA23–3小鼠的肠系膜淋巴结（MLN）的OVA特异性CD4 ⁺ T细胞与来自BALB / cA小鼠的MLN的CD11c ⁺树突状细胞一起培养。然后CD69 ⁺的CD69表达水平和细胞因子产生水平测量T细胞以评估活化。通过饲喂含或不含MBP的OVA饮食（OVA饮食）28 d来评估补充OVA 23–3小鼠的MBP的效果。测量肠道炎症，以及MLN对CD4 ⁺ T细胞的激活和细胞因子的产生，以及股骨矿物质密度。在体外培养中，MBP抑制CD4 ⁺ T细胞过度活化和IL-4产生。在OVA饮食中添加MBP可以减少由OVA饮食喂养的OVA23-3小鼠产生的OVA特异性IgE的产生，并稍微解决由CD4 ⁺产生的过量IL-4引起的发展性肠病。T细胞。在OVA23–3小鼠中喂OVA饮食会导致骨质疏松，并伴有肠病，而MBP补充剂可在小鼠中防止骨质流失并增加破骨细胞形成抑制因子骨保护素。MBP补充剂对MLN和骨髓中T细胞活化的抑制作用可能有助于防止由于过多的IL-4产生和伴有肠病的骨质流失引起的IgE水平升高。我们的发现表明，MBP可能有助于减轻T细胞相关的炎症和骨质流失。