Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self‐assembled DNA nanobarrel that scaffolds a nanodisc‐like lipid environment. Compared with the membrane‐scaffolding‐protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins.

中文翻译：

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将DNA折叠到脂质缀合的纳米桶中，以控制膜蛋白的重组。

在DNA折纸技术的基础上，我们介绍了一种将单个膜蛋白重构为自组装的DNA纳米桶的方法，该纳米桶支撑着类似于纳米光盘的脂质环境。与膜支架蛋白纳米碟技术相比，我们的方法产生了确定的化学计量比，可控制的大小以及增强的膜蛋白重构稳定性和同质性。我们进一步证明了DNA纳米桶在膜蛋白结构分析中的潜在应用。