The common language of bacteria and higher life forms is a lexicon of small molecules that the research community is only beginning to decipher. While many new signaling molecules have been discovered in recent years, the identification of their targets is mostly lagging. This review will focus on the latest chemical-probe based research aimed at understanding how bacteria interact chemically with mammals and plants. In general, chemical biology strategies remain under-utilized in this complex field of research, with a few key exceptions, and we hope that this review encourages others to implement these techniques in their research. Specifically, we highlight the chemical biology techniques used in recent studies, especially activity-based protein profiling, that have been applied to unravel the chemical mechanisms of interkingdom interactions.

中文翻译：

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揭示细菌-真核信号转导的化学策略

细菌和高级生命形式的通用语言是小分子的词典，研究界才开始对其进行解读。尽管近年来发现了许多新的信号传导分子，但对它们的靶标的鉴定大多滞后。本文将重点关注基于化学探针的最新研究，旨在了解细菌如何与哺乳动物和植物进行化学相互作用。一般而言，化学生物学策略在这一复杂的研究领域中仍未得到充分利用，但有一些关键的例外，我们希望这篇综述鼓励其他人在他们的研究中实施这些技术。具体而言，我们重点介绍了最近研究中使用的化学生物学技术，尤其是基于活性的蛋白质谱分析，这些技术已被用于阐明相互间相互作用的化学机理。