Scope determination: Key residues accounting for different activities of CYP154F1 and its thoroughly characterized relative CYP154F1 were identified based on a structural alignment of their homology models. Subsequently, site‐directed mutagenesis was applied to extend the substrate spectrum of CYP154F1 and increase its activity.

中文翻译：

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CYP154F1的定型突变及其从底物谱扩展的定点诱变

范围的确定：根据CYP154F1的同源性模型的结构比对，确定了CYP154F1不同活性的关键残基及其充分表征的相对CYP154F1。随后，应用定点诱变来扩展CYP154F1的底物谱并增加其活性。