Abstract A multilayered polylactic acid (PLA)/halloysite (HAL) porous membrane encapsulated with aminoglycoside antibiotic (gentamicin) was prepared by electrospinning technique. The prepared electrospun membrane displayed smooth morphology and uniform fiber distribution, while various layers of PLA mats were tightly bounded together. Significant enhancement in mechanical properties and stability was observed in membranes reinforced by HAL and the multilayered membrane loaded with gentamicin, while the drug loaded membrane showed no delamination. In addition, incorporation of HAL led to improvement in thermal stability of the PLA nanofibrous membranes. The in vitro drug release study of gentamicin loaded membrane initially shows a burst release of gentamicin, followed by slow rate of drug release up to 48 h. Antimicrobial efficacy of electrospun membrane was tested on a gram-positive (Staphylococcus aureus) and a gram-negative (Escherichia coli) bacteria, while results indicated that the gentamicin released from electrospun membrane has retained its antibacterial activity. These results signified the potential of multilayered porous PLA/HAL membranes to be utilized in prevention of infection in bone regeneration applications.

中文翻译：

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纳米纤维中的纳米管：用于骨再生应用的抗菌多层聚乳酸/埃洛石/庆大霉素膜

摘要 采用静电纺丝技术制备了氨基糖苷类抗生素（庆大霉素）包裹的多层聚乳酸（PLA）/埃洛石（HAL）多孔膜。制备的电纺膜显示出光滑的形态和均匀的纤维分布，而各层 PLA 垫紧密结合在一起。在由 HAL 增强的膜和装载庆大霉素的多层膜中观察到机械性能和稳定性的显着增强，而装载药物的膜没有分层。此外，HAL 的加入提高了 PLA 纳米纤维膜的热稳定性。载有庆大霉素的膜的体外药物释放研究最初显示庆大霉素突然释放，随后药物释放速度缓慢，最长可达 48 小时。在革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌）细菌上测试了电纺膜的抗菌功效，而结果表明从电纺膜释放的庆大霉素保留了其抗菌活性。这些结果表明多层多孔 PLA/HAL 膜在预防骨再生应用中的感染方面具有潜力。