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Lupin seed γ-conglutin: Extraction and purification methods - A review
Trends in Food Science & Technology ( IF 15.3 ) Pub Date : 2017-12-19 , DOI: 10.1016/j.tifs.2017.12.008
Sharmilee P. Mane , Stuart K. Johnson , Marcello Duranti , Vishnu K. Pareek , Ranjeet P. Utikar

Background

Lupin, the largest legume crop in Australia, is gaining global attention because of its unique protein γ-conglutin, which has shown promise as a nutraceutical for controlling blood glucose level and thus reducing the risk of type II diabetes development. Type II diabetes is a chronic condition affecting millions of individuals worldwide, which urgently requires natural side effect free therapies as alternatives to currently used drugs. Purification of γ-conglutin opens up a new avenue for high-value products from lupin seeds as nutraceuticals, the market for which is predicted to reach US$ 250 billion by 2018 (Dutta, Mahabir, & Pathak, 2013).

Scope and approach

Previously, several research groups have reported trials on extracting and purifying proteins from lupin seed. However, most of these methods have focussed on protein isolates as food ingredients. Very few reports have aimed to purify γ-conglutin from the total proteins, but the methods reported are time-consuming and unsuitable for commercial scale production of high purity γ-conglutin due to the involvement of many processing steps for nutraceutical application. Hence there is a need to fully understand all reported γ-conglutin extraction and purification processes in terms of their advantages and limitations, so that an effective scalable purification process for nutraceutical grade γ-conglutin may be designed in the future.

Key findings and conclusions

This article reviews reported extraction and purification methods for γ-conglutin, to provide a basis for the development of novel purification technique/process for this potentially highly valuable protein.



中文翻译:

羽扇豆种子γ-凝集素:提取和纯化方法-审查

背景

羽扇豆是澳大利亚最大的豆类作物,由于其独特的蛋白质γ-凝集素而备受全球关注,该蛋白已显示出有望作为控制血糖水平的营养食品,从而降低II型糖尿病发展的风险。II型糖尿病是一种慢性病,影响全世界数以百万计的人,因此迫切需要使用无副作用的天然疗法作为当前使用药物的替代品。γ-凝集素的纯化为羽扇豆种子作为保健品的高价值产品开辟了一条新途径,预计到2018年其市场规模将达到2500亿美元(Dutta,Mahabir和Pathak,2013年)。

范围和方法

以前,几个研究小组已经报告了从羽扇豆种子中提取和纯化蛋白质的试验。但是,这些方法大多数都集中在分离蛋白作为食品成分上。很少有报道旨在从总蛋白中纯化γ-凝集素,但由于涉及营养保健品的许多加工步骤,所报道的方法既费时又不适合高纯度γ-凝集素的工业规模生产。因此,有必要就其优点和局限性充分了解所有报道的γ-粘连蛋白的提取和纯化方法,以便将来可以设计出一种适用于营养级γ-粘连蛋白的有效可扩展纯化方法。

主要发现和结论

本文综述了报道的γ-凝集素的提取和纯化方法,为开发这种潜在有价值的蛋白质的新型纯化技术/方法提供了基础。

更新日期:2017-12-19
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