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Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)
Analytical Methods ( IF 3.1 ) Pub Date : 2017-12-18 00:00:00 , DOI: 10.1039/c7ay02506h
N. M. El Zahar 1, 2, 3, 4, 5 , N. Magdy 1, 2, 3, 4, 5 , A. M. El-Kosasy 1, 2, 3, 4, 5 , Wayne D. Beck 6, 7, 8, 9, 10 , Patrick M. Callahan 6, 7, 8, 9, 10 , Alvin V. Terry 6, 7, 8, 9, 10 , Michael G. Bartlett 10, 11, 12, 13, 14
Affiliation  

A selective and sensitive method was developed to simultaneously quantify tianeptine and its main active metabolite tianeptine MC5 in rat plasma and brain tissue. A one-step liquid–liquid extraction was developed for sample preparation. The linear range for plasma was from 1.0–500.0 ng mL−1 and for brain tissue it was from 1.0–500.0 ng g−1. The method was validated in accordance with US FDA guidelines (precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE) with a consistent recovery). Stable isotope-labeled tianeptine and tianeptine MC5 (tianeptine-D4 and tianeptine MC5-D4) were used as the internal standards achieving good reproducibility while reducing the matrix effects. This method was successfully applied to a preclinical study for the simultaneous determination of tianeptine and tianeptine MC5 in rat plasma and brain tissue.

中文翻译:

高效液相色谱/电喷雾电离串联质谱法(LC-ESI-MS / MS)同时测定大鼠血浆和脑组织中的田汀及其活性代谢物田肽MC5

开发了一种选择性和灵敏的方法,可同时定量大鼠血浆和脑组织中的田间汀及其主要活性代谢物田间汀MC5。为样品制备开发了一种单步液-液萃取方法。血浆的线性范围是1.0–500.0 ng mL -1,而脑组织的线性范围是1.0–500.0 ng g -1。该方法已根据美国FDA指南进行了验证(精度在15%相对标准偏差(RSD)之内,准确度在15%相对误差(RE)之内,并且回收率稳定。稳定的同位素标记的替丁汀和替丁汀MC5(替丁汀-D4和替丁汀MC5-D4)用作内标,可实现良好的重现性,同时降低了基质效应。该方法已成功应用于临床前研究,可同时测定大鼠血浆和脑组织中的田间汀和田间汀MC5。
更新日期:2017-12-18
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