There is a significant need for new biodegradable protein stabilizing polymers. Herein, the synthesis of a polymer with trehalose side chains and hydrolytically degradable backbone esters and its evaluation for protein stabilization and cytotoxicity are described. Specifically, an alkene‐containing parent polymer is synthesized by reversible addition–fragmentation chain transfer polymerization, and thiolated trehalose is installed using a radical‐initiated thiol–ene reaction. The stabilizing properties of the polymer are investigated by thermally stressing granulocyte colony‐stimulating factor (G‐CSF), which is expressed and purified using a custom‐designed G‐CSF fusion protein with a polyhistidine‐tagged maltose binding protein. The degradable polymer is shown to stabilize G‐CSF to 66% after heating at 40 °C. Poly(5,6‐benzo‐2‐methylene‐1,3‐dioxepane (BMDO)‐co‐butyl methacrylate‐trehalose) is degraded and its cellular compatibility is investigated. While the polymer is noncytotoxic, cytotoxic effects are observed from the degraded products in fibroblasts and murine myeloblasts. These data provide important information for future use of BMDO‐containing trehalose glycopolymers for biomedical applications.

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RAFT聚合可降解海藻糖聚合物的合成及生物学评价

迫切需要新型可生物降解的蛋白质稳定聚合物。本文描述了具有海藻糖侧链和可水解降解主链酯的聚合物的合成及其对蛋白质稳定性和细胞毒性的评估。具体来说，通过可逆加成断裂链转移聚合合成含烯烃的母体聚合物，并使用自由基引发的硫醇-烯反应安装硫醇化海藻糖。通过热应激粒细胞集落刺激因子 (G-CSF) 研究聚合物的稳定性，该因子使用定制设计的 G-CSF 融合蛋白与多组氨酸标记的麦芽糖结合蛋白进行表达和纯化。在 40 °C 加热后，可降解聚合物可将 G-CSF 稳定至 66%。聚（5,6-苯并-2-亚甲基-1,3-二氧杂环庚烷（BMDO）-甲基丙烯酸丁酯-海藻糖）被降解，并研究了其细胞相容性。虽然该聚合物无细胞毒性，但在成纤维细胞和鼠成髓细胞中的降解产物中观察到细胞毒性作用。这些数据为未来将含有 BMDO 的海藻糖糖聚合物用于生物医学应用提供了重要信息。