The species of Agrimonia and Filipendula have been traditionally used in folk medicine as anti-inflammatory herbs. This study extends the knowledge on bioactivities of F. palmata, A. eupatoria, A. procera, F. ulmaria and F. vulgaris by comprehensive characterization of their methanolic extracts. Antioxidant properties of extracts were evaluated by DPPH^• (2,2-diphenyl-1-picrylhydrazyl), ABTS^•+ 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) scavenging and oxygen radical absorbance capacities (ORAC). Genotoxicity of extracts was tested using alkaline single-cell gel electrophoresis (comet) and cytokinesis-block micronucleus assays in human lymphocytes in vitro and the Ames Salmonella/microsome test. All investigated Agrimonia and Filipendula extracts possessed strong antioxidant activity, which was comparable with that of a standard antioxidant trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). Thirty five compounds belonging to the classes of phenolic acids, flavonoids, phenylpropanoids and ellagitanins were detected by ultra-performance liquid chromatography – mass spectrometry (UPLC-Q-TOF-MS). Agrimonia and Filipendula extracts induced an increase in a DNA damage in the comet assay expressed as mean percentage of DNA in the comet tail. However, these extracts did not produce reverse mutation in bacterial cells in the Ames test and were not genotoxic in the micronucleus test. However, a slight though significant decrease of nuclear division index values was determined. In general, this study proved that Agrimonia and Filipendula species are a good source of bioactive compounds; their extracts may be classified as non-mutagenic and non-clastogenic in vitro under conditions of the current study. Consequently, the plants may be a promising material for nutraceuticals and natural medicines.

仙鹤草和菲律宾菊属植物传统上已在民间医学中用作消炎药草。这项研究通过全面表征其甲醇提取物，扩展了对棕榈果，中型农杆菌，procera，普鲁姆氏菌和寻常果的生物活性的认识。提取物的抗氧化特性通过DPPH ^•（2,2-二苯基-1-吡啶并肼基），ABTS ^•+进行评估2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）清除能力和氧自由基吸收能力（ORAC）。使用碱性单细胞凝胶电泳（彗星）和胞质分裂阻滞微核测定法在人淋巴细胞中体外和Ames沙门氏菌/微粒体测试中测试提取物的基因毒性。所有研究过的仙鹤草和菲律宾菊提取物均具有很强的抗氧化活性，可与标准抗氧化剂trolox（6-羟基-2,5,7,8-四甲基苯并-2-羧酸）相媲美。通过超高效液相色谱-质谱法（UPLC-Q-TOF-MS）检测到了35种属于酚酸，类黄酮，苯基丙烷和鞣花素的化合物。仙鹤草和菲律宾菊提取物在彗星试验中诱导了DNA损伤的增加，表示为彗尾中DNA的平均百分比。但是，这些提取物在Ames试验中并未在细菌细胞中产生反向突变，并且在微核试验中没有遗传毒性。但是，确定了核分裂指数值略有下降，但有明显下降。总的来说，这项研究证明了仙鹤草和菲律宾菊是生物活性化合物的良好来源。在当前研究条件下，其提取物在体外可分为非致突变性和非致死性。因此，植物可能是有前景的保健食品和天然药物。