Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation.

中文翻译：

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固定在硅胶整体上的脂肪酶作为用于甘油三酸酯酯交换的连续流微反应器†

已经制备了固定在二氧化硅单块上的脂肪酶，并将其用作生物催化连续流微反应器，用于将三丁酸甘油酯作为模型生物油组分进行酯交换反应。南极假丝酵母脂肪酶被捕集在二氧化硅整体结构的孔中，其成功的固定化是由丁酸4-硝基苯酯水解为4-硝基苯酚来证明的。固定在二氧化硅单块上的脂肪酶在室温下对三丁酸甘油酯的酯交换反应具有活性，其反应性是甲醇：三丁酸甘油酯比例，流速，温度和质构性质的函数。具有高表面积和大中孔和大孔通道的整体结构通过改善分子扩散增强了酯交换活性。最佳的固定化脂肪酶微反应器在30°C下持续100 h以上几乎没有定量失活酯。