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Controllable extension of hairpin-structured flaps to allow low-background cascade invasive reaction for a sensitive DNA logic sensor for mutation detection†
Chemical Science ( IF 8.4 ) Pub Date : 2017-12-14 00:00:00 , DOI: 10.1039/c7sc04210h
Yun-Long Liu 1, 2, 3, 4, 5 , Hai-Ping Wu 4, 5, 6 , Qiang Zhou 5, 7, 8, 9 , Qin-Xin Song 5, 7, 8, 9 , Jian-Zhong Rui 1, 2, 3, 4, 5 , Xiao-Xiang Guan 1, 2, 3, 4, 5 , Guo-Hua Zhou 1, 2, 3, 4, 5 , Bing-Jie Zou 1, 2, 3, 4, 5
Affiliation  

A DNA logic sensor was constructed for gene mutation analysis based on a novel signal amplification cascade by controllably extending a hairpin-structured flap to bridge two invasive reactions. The detection limit was as low as 0.07 fM, and the analytical specificity is high enough to unambiguously pick up 0.02% mutants from a large amount of wild-type DNA. Gene mutations related to the personalized medicine of gefitinib, a typical tyrosine kinase inhibitor, were analyzed by the DNA logic sensor with only a 15 minute response time. Successful assay of tissue samples and cell-free plasma DNA indicates that the new concept we proposed here could benefit clinicians for straightforward prescription of a mutation-targeted drug.

中文翻译:

发夹结构皮瓣的可控制延伸,允许低背景级联入侵反应,用于敏感的DNA逻辑传感器进行突变检测

通过可控地扩展发夹结构的皮瓣来桥接两个侵入性反应,基于新型信号放大级联构建了用于基因突变分析的DNA逻辑传感器。检测限低至0.07 fM,并且分析特异性很高,足以从大量的野生型DNA中毫无疑问地获得0.02%的突变体。DNA逻辑传感器仅用15分钟的响应时间即可分析与吉非替尼(一种典型的酪氨酸激酶抑制剂)的个性化药物相关的基因突变。对组织样品和无细胞血浆DNA的成功分析表明,我们在此提出的新概念可能会有益于临床医生直接针对突变靶向药物开具处方。
更新日期:2017-12-14
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