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Effect of ultrasound on size, morphology, stability and antioxidant activity of selenium nanoparticles dispersed by a hyperbranched polysaccharide from Lignosus rhinocerotis
Ultrasonics Sonochemistry ( IF 8.4 ) Pub Date : 2017-12-14 , DOI: 10.1016/j.ultsonch.2017.12.022
Wenfei Cai , Ting Hu , Amr M. Bakry , Zhaomin Zheng , Yidong Xiao , Qilin Huang

The differences between ultrasonic and non-ultrasonic approaches in synthesizing Lignosus rhinocerotis polysaccharide-selenium nanoparticles (LRP-SeNPs) were compared in terms of size, morphology, stability and antioxidant activity by UV-VIS, FT-IR, X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) with high resolution TEM. Results indicated that the SeNPs were associated with the LRP macromolecules in a physical adsorption pattern without breaking chemical bonds, and the ultrasonic treatment reduced the size of SeNPs, narrowed the size distribution as well as improved the stability. Due to the LRP compact coil structure loosed under ultrasonic cavitation, the SeNPs could be easily diffused into the LRP internal branches instead of gathering on the LRP surface and were well dispersed and eventually stabilized throughout the extended branches. After ultrasound treatment, the SeNPs had a minimum average diameter of ∼50 nm and the LRP-SeNPs could remain homogeneous and translucent for 16 days within 200 nm size. Furthermore, the ultrasound-treated LRP-SeNPs exhibited higher DPPH and ABTS radical-scavenging abilities than those untreated with ultrasound. This difference may be attributed to the reason that ultrasound can reduce the SeNPs size and increase the specific surface area, which provides sufficient active sites to react with the free radicals and suppress the oxidizing reactions. The integrated results demonstrated that ultrasound played a crucial role in the dispersion, size control, stabilization and antioxidant activity of SeNPs.



中文翻译:

超声波对木犀牛超支化多糖分散硒纳米颗粒尺寸,形貌,稳定性和抗氧化活性的影响

超声和非超声方法在合成木犀牛中的区别通过UV-VIS,FT-IR,X射线衍射(XRD),动态光散射(DLS),透射电子显微镜(%)对多糖-硒纳米颗粒(LRP-SeNPs)的大小,形态,稳定性和抗氧化活性进行了比较( TEM)和具有高分辨率TEM的能量色散X射线(EDX)。结果表明,SeNPs与LRP大分子以物理吸附方式结合而没有破坏化学键,并且超声处理减小了SeNPs的尺寸,收窄了尺寸,并提高了稳定性。由于LRP紧凑的线圈结构在超声空化作用下松散,因此SeNPs可以很容易地扩散到LRP内部分支中,而不是聚集在LRP表面上,并且可以很好地分散并最终稳定在整个延伸的分支中。超声处理后,SeNPs的最小平均直径约为50 nm,LRP-SeNPs在200 nm的尺寸范围内可以保持均匀和半透明状态达16天。此外,与未经超声处理的LRP-SeNPs相比,经超声处理的LRP-SeNPs具有更高的DPPH和ABTS自由基清除能力。这种差异可以归因于超声波可以减小SeNPs大小并增加比表面积的原因,超声波可以提供足够的活性位点与自由基反应并抑制氧化反应。综合结果表明,超声在SeNPs的分散,尺寸控制,稳定化和抗氧化活性中起着至关重要的作用。此外,与未经超声处理的LRP-SeNPs相比,经超声处理的LRP-SeNPs具有更高的DPPH和ABTS自由基清除能力。这种差异可以归因于超声波可以减小SeNPs大小并增加比表面积的原因,超声波可以提供足够的活性位点与自由基反应并抑制氧化反应。综合结果表明,超声在SeNPs的分散,尺寸控制,稳定化和抗氧化活性中起着至关重要的作用。此外,与未经超声处理的LRP-SeNPs相比,经超声处理的LRP-SeNPs具有更高的DPPH和ABTS自由基清除能力。这种差异可以归因于超声波可以减小SeNPs大小并增加比表面积的原因,超声波可以提供足够的活性位点与自由基反应并抑制氧化反应。综合结果表明,超声在SeNPs的分散,尺寸控制,稳定化和抗氧化活性中起着至关重要的作用。它提供足够的活性位点与自由基反应并抑制氧化反应。综合结果表明,超声在SeNPs的分散,尺寸控制,稳定化和抗氧化活性中起着至关重要的作用。它提供足够的活性位点与自由基反应并抑制氧化反应。综合结果表明,超声在SeNPs的分散,尺寸控制,稳定化和抗氧化活性中起着至关重要的作用。

更新日期:2017-12-15
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