The photosynthetic light-harvesting–reaction center core complex (LH1-RC) is a natural excitonic and photovoltaic device embedded in a lipid membrane. In order to apply LH1-RCs as a biohybrid energy-producing material, some important issues must be addressed, including how to make LH1-RCs function as efficiently as possible. In addition, they should be characterized to evaluate how many active LH1-RCs efficiently work in artificial systems. We report here that an anionic phospholipid, phosphatidylglycerol (PG), stabilizes the charge-separated state (a photooxidized electron donor and reduced quinone pair, P⁺Q_B^–) of LH1-RC (from Rhodopseudomonas palustris) and enhances its activity in photocurrent generation. Steady-state fluorometric analysis demonstrated that PG enhances the formation of the P⁺Q_B^– state at lower irradiances. The photocurrent generation activity was analyzed via Michaelis–Menten kinetics, revealing that 38% of LH1-RCs reconstituted into the PG membrane generated photocurrent at a turnover frequency of 46 s^–1. PG molecules, which interact with LH1-RC in vivo, play the role of an active effector component for LH1-RC to enhance its function in the biohybrid system.

中文翻译：

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类脂红假单胞菌的光分离反应中心核心复合物（LH1-RC）的脂质控制的电荷分离态（P ⁺ Q _B ^–）的稳定化和光电流产生活性。

光合光捕获-反应中心核心复合物（LH1-RC）是嵌入脂质膜中的天然激子和光电器件。为了将LH1-RCs用作生物杂交能量产生材料，必须解决一些重要问题，包括如何使LH1-RCs尽可能有效地发挥作用。此外，应该对它们进行表征，以评估在人造系统中有效工作的有源LH1-RC数量。我们在这里报告，阴离子磷脂，磷脂酰甘油（PG），稳定了LH1-RC（来自Rhodopseudomonas palustris）的电荷分离状态（光氧化的电子供体和还原的醌对，P ⁺ Q _B ^–））并增强其在光电流产生中的活性。稳态荧光分析表明，PG提高P的形成⁺ Q_乙^-在较低的辐照度的状态。通过Michaelis-Menten动力学分析了光电流的产生活性，发现重构到PG膜中的38％的LH1-RCs以46 s ^–1的周转频率产生了光电流。在体内与LH1-RC相互作用的PG分子充当LH1-RC的活性效应子组分，以增强其在生物杂交系统中的功能。