Benign prostate hyperplasia (BPH) is a common age-related health problem affecting almost 3 out of 4 men in their sixties. Chrysin is a dietary phytoestrogen found naturally in bee propolis and various plant extracts. It possesses antioxidant, anti-inflammatory and anti-proliferative properties. The current study was conducted to explore the role chrysin plays in protection against testosterone-induced BPH in rats. On grounds of a preliminary experiment, a dose of chrysin (50 mg/kg) was chosen for further investigation. Testosterone significantly depleted glutathione, suppressed superoxide dismutase and catalase activities, and elevated lipid peroxidation. Moreover, it markedly scaled down the level of cleaved caspase-3 enzyme, reduced Bax/Bcl-2 ratio and mRNA expression of p53 and p21; conversely, protein expression of proliferating cell nuclear antigen was enhanced. Chrysin alleviated testosterone-induced oxidative stress and restored cleaved caspase-3 level, Bax/Bcl-2 ratio and mRNA expression of p53 and p21 to almost control levels. Chrysin prevented the increase in binding activity of nuclear factor kappa B (NF-κB) p65 subunit, mRNA expression of insulin-like growth factor 1 (IGF-1) and insulin-like growth factor 1 receptor (IGF-1R). These data highlight the protective role of chrysin against experimentally-induced BPH. This is attributed - at least partly - to its antioxidant, antiproliferative and proapoptotic properties.

良性前列腺增生（BPH）是一个常见的与年龄相关的健康问题，在60多岁的男性中影响了近四分之三。菊花蛋白是一种饮食植物雌激素，天然存在于蜂胶和各种植物提取物中。它具有抗氧化，抗炎和抗增殖的特性。进行当前的研究是为了探索菊花素在保护大鼠抵抗睾丸激素诱导的BPH中的作用。根据初步实验，选择了一定剂量的菊花蛋白（50 mg / kg）用于进一步研究。睾酮显着减少了谷胱甘肽，抑制了超氧化物歧化酶和过氧化氢酶的活性，并增加了脂质过氧化作用。此外，它显着降低了裂解的caspase-3酶的水平，降低了Bax / Bcl-2比率以及p53和p21的mRNA表达。反过来，增殖细胞核抗原的蛋白表达增强。Chrysin减轻了睾丸激素诱导的氧化应激，并恢复了裂解的caspase-3水平，Bax / Bcl-2比率以及p53和p21的mRNA表达至几乎控制水平。胰蛋白酶阻止了核因子κB（NF-κB）p65亚基的结合活性，胰岛素样生长因子1（IGF-1）和胰岛素样生长因子1受体（IGF-1R）mRNA的表达增加。这些数据强调了菊花素对实验诱导的BPH的保护作用。这至少部分归因于其抗氧化，抗增殖和促凋亡特性。胰蛋白酶阻止了核因子κB（NF-κB）p65亚基的结合活性，胰岛素样生长因子1（IGF-1）和胰岛素样生长因子1受体（IGF-1R）mRNA的表达增加。这些数据强调了菊花素对实验诱导的BPH的保护作用。这至少部分归因于其抗氧化，抗增殖和促凋亡特性。胰蛋白酶阻止了核因子κB（NF-κB）p65亚基的结合活性，胰岛素样生长因子1（IGF-1）和胰岛素样生长因子1受体（IGF-1R）mRNA的表达增加。这些数据强调了菊花素对实验诱导的BPH的保护作用。这至少部分归因于其抗氧化，抗增殖和促凋亡特性。