Objectives

Smear layer deproteinizing was proved to reduce the organic phase of smear layer covered on dentin surface. It was shown to eliminate hybridized smear layer and nanoleakage expression in resin–dentin bonding interface of two-step self-etch adhesive. This study aimed to investigate those effects on various one-step self-etch adhesives.

Methods

Four different one-step self-etch adhesives were used in this study; SE One (SE), Scotchbond™ Universal (SU), BeautiBond Multi (BB), and Bond Force (BF). Flat human dentin surfaces with standardized smear layer were prepared. Smear layer deproteinizing was carried out by the application of 50 ppm hypochlorous acid (HOCl) on dentin surface for 15 s followed by Accel^® (p-toluenesulfinic acid salt) for 5 s prior to adhesive application. No surface pretreatment was used as control. Microtensile bond strength (μTBS) and nanoleakage under TEM observation were investigated. The data were analyzed by two-way ANOVA and Tukey’s post-hoc test and t-test at the significant level of 0.05.

Results

Smear layer deproteinizing significantly improved μTBS of SE, SU, and BB (p < 0.001). Hybridized smear layer observed in control groups of SE, BB, and BF, and reticular nanoleakage presented throughout the hybridized complex in control groups of BB and BF were eliminated upon the smear layer deproteinizing.

Significance

Smear layer deproteinizing by HOCl and Accel^® application could enhance the quality of dentin for bonding to one-step self-etch adhesives, resulting in the improving μTBS, eliminating hybridized smear layer and preventing reticular nanoleakage formation in resin–dentin bonding interface.

中文翻译：

---

涂抹层去蛋白化可改善一步式自蚀刻粘合剂与牙本质的粘合

目标

涂片层去蛋白被证明可以减少覆盖在牙本质表面上的涂片层的有机相。它显示出消除了两步自蚀刻胶粘剂在树脂-牙本质粘合界面中的混杂涂片层和纳米泄漏的表达。这项研究旨在研究这些对各种单步自蚀刻胶粘剂的影响。

方法

在这项研究中使用了四种不同的单步自蚀刻胶粘剂。SE One（SE），Scotchbond™Universal（SU），BeautiBond Multi（BB）和Bond Force（BF）。制备具有标准化涂片层的平坦人牙本质表面。玷污层脱蛋白处理用的50ppm的次氯酸（HOCl）的牙本质表面上施加15秒，然后加速进行^®（p -toluenesulfinic乙酸盐）5秒前粘合剂应用。没有将表面预处理用作对照。研究了TEM观察下的微拉伸粘合强度（μTBS）和纳米漏失。通过双向ANOVA和Tukey的事后检验和t检验对数据进行了分析，显着性水平为0.05。

结果

涂抹层去蛋白化显着改善了SE，SU和BB的μTBS（p  <0.001）。SE，BB和BF对照组中观察到的杂交涂片层以及BB和BF对照组中整个杂交复合物中出现的网状纳米泄漏在涂片层脱蛋白后被消除。

意义

玷污层由次氯酸和加速脱蛋白^®应用可增强牙本质的粘接质量一步法自蚀刻粘合剂，导致改善μTBS，消除杂交玷污层和防止树脂牙本质粘合界面网状纳米渗漏形成。