Fructus Psoraleae, a traditional Chinese medicine, is widely used for preventing and treating various diseases such as vitiligo, osteoporosis and psoriasis. Coumarin, such as psoralenoside, isopsoralenoside, psoralen and isopsoralen, are important compounds in Fructus Psoraleae. In our study, ultra performance liquid chromatography coupled with diode array detector was employed for an excellent method validation for simultaneous quantification of psoralenoside, isopsoralenoside, psoralen and isopsoralen, which was further applied in performing general survey of Fructus Psoraleae from the different origins and chemical identification of the roasted from raw Fructus Psoraleae in the light of illuminating the transformed rule of psoralenoside and isopsoralenoside. There is a reciprocal relationship between (iso)psoralenoside and (iso)psoralen, and the total content remains balance in Fructus Psoraleae from the different origins. In addition, we found that (iso)psoralenoside in the powder of the raw Fructus Psoraleae could be easily transformed into (iso)psoralen in methanol aqueous solution, especially above 50% water, rather than the roasted one. Thus, we proposed a hypothesis that transformation between (iso)psoralenoside and (iso)psoralen was hindered by inactivation of β-glucosidase in the process of roasting Fructus Psoraleae, which was further verified by observing transformation of (iso)psoralenoside under the different conditions, such as temperature, pH and β-glucosidase. Therefore, we developed a feasible method to distinguish the roasted from raw Fructus Psoraleae by observing conversion from (iso)psoralenoside to (iso)psoralen in 50% methanol aqueous solution. In summary, these results pave the way for elevating quality standard for Fructus Psoraleae and distinguishing the salt-processed from raw Fructus Psoraleae.

中文翻译：

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不同产地补骨脂的普查及生补骨脂焙烧物的化学鉴定

补骨脂是一种中药，广泛用于防治白癜风、骨质疏松症、牛皮癣等多种疾病。香豆素，如补骨脂苷、异补骨脂苷、补骨脂素和异补骨脂素，是补骨脂中的重要化合物。在我们的研究中，超高效液相色谱结合二极管阵列检测器被用于同时定量补骨脂苷、异补骨脂苷、补骨脂素和异补骨脂素的优良方法验证，并进一步应用于不同来源补骨脂的普查和化学鉴定。根据补骨脂苷与异补骨脂苷的转化规律，对生补骨脂进行焙烧。(iso)psoralenoside 和 (iso)psoralen 之间存在相互关系，不同产地补骨脂总含量保持平衡。此外，我们发现生补骨脂粉末中的（异）补骨脂素在甲醇水溶液中，特别是在50%以上的水份中，比经过烘烤的更容易转化为（异）补骨脂素。因此，我们提出了一个假设，即（异）补骨脂苷和（异）补骨脂素之间的转化受到补骨脂烘烤过程中β-葡萄糖苷酶失活的阻碍，并通过观察不同条件下（异）补骨脂苷的转化进一步验证了这一假设。 ，如温度、pH 值和 β-葡萄糖苷酶。因此，我们开发了一种可行的方法，通过观察在 50% 甲醇水溶液中从（异）补骨脂苷到（异）补骨脂素的转化来区分烤补骨脂和生补骨脂。总之，