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Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite†
Metallomics ( IF 3.4 ) Pub Date : 2017-12-13 00:00:00 , DOI: 10.1039/c7mt00285h
S. Meyer 1, 2, 3, 4, 5 , A. López-Serrano 4, 6, 7 , H. Mitze 1, 2, 3, 4 , N. Jakubowski 4, 6, 7 , T. Schwerdtle 1, 2, 3, 4, 5
Affiliation  

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0–48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.

中文翻译:

ICP-MS / MS进行单细胞分析,可作为砷亚铁细胞生物利用度研究的快速工具

单细胞电感耦合等离子体质谱法(SC-ICP-MS)已成为一种功能强大且快速的工具,可以在单细胞水平上评估元素组成。在这项研究中,将细胞重悬于水中后,首次通过SC-ICP-MS / MS成功评估了砷的细胞生物利用度(25和50μM孵育0-48小时)。该程序避免了由细胞固定方法(例如甲醇固定)引起的正常发生的细胞膜通透性。这种SC-ICP-MS / MS方法的可靠性和可行性(每细胞检测限为0.35 fg)已通过细胞消化以及硫和磷的平行测量后的常规批量ICP-MS / MS分析得到验证。
更新日期:2017-12-13
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