Interleukin 8 (IL-8), also known as C-X-C motif ligand 8(CXCL8), is a proinflammatory chemokine functioned in neutrophil chemotaxis and activation. And it plays an important role in the process of glioma stem-like cell vascularization in the latest research. Herein, a dual-function microfluidic biosensor based on rolling circle amplification (RCA) was fabricated for cell culture and online IL-8 detection. A microfluidic chip was designed with two high passages connected by the vertical channels. One of the channels with immobilized capture antibody was prepared for IL-8 detection and another channel for cell culture. Immunoassays were achieved by a sandwich structure consisting of antibodies, IL-8, and aptamers. Signal amplification was mainly due to RCA and biotin-streptavidin linkage. The linear range for IL-8 was 7.5 −120 pg mL⁻¹ in this assay. Moreover, the developed method was successfully applied to detect the IL-8 in tumor-derived endothelial cells (TDEC) and Human Umbilical Vein Endothelial cells (HUVEC) under chemical hypoxia condition. Semi-quantitative detection of IL-8 consumption in HUVEC cells in low oxygen condition was also achieved. These results were in statistical agreement with those obtained by commercial assay of enzyme-linked immunoassay kit (ELISA). The microfluidic chip based biosensor reported hereby has a large prospect in the basic research and clinical diagnosis of cancer stem cell.

白介素8（IL-8），也称为CXC基序配体8（CXCL8），是一种在中性粒细胞趋化和激活中起作用的促炎趋化因子。在最新研究中，它在神经胶质瘤干细胞样血管形成过程中起着重要作用。在这里，双功能微流控生物传感器基于滚环扩增（RCA）制作用于细胞培养和在线IL-8检测。设计了一种微流体芯片，该芯片具有通过垂直通道连接的两个高通道。制备具有固定化捕获抗体的通道之一用于IL-8检测，另一通道用于细胞培养。免疫测定是通过由抗体，IL-8和适体组成的夹心结构实现的。信号放大主要是由于RCA和生物素-链霉亲和素连接。IL-8的线性范围是7.5 -120 pg mL在该测定法中为^-1。此外，所开发的方法已成功地用于检测化学低氧条件下肿瘤来源的内皮细胞（TDEC）和人脐静脉内皮细胞（HUVEC）中的IL-8。还实现了在低氧条件下对HUVEC细胞中IL-8消耗量的半定量检测。这些结果与通过酶联免疫测定试剂盒（ELISA）的商业测定获得的结果在统计上吻合。本文报道的基于微流控芯片的生物传感器在癌症干细胞的基础研究和临床诊断中具有广阔的前景。