Protein PEGylation plays an important role in the whole panel of reactions used for the modification and improvement of therapeutic proteins. The classic production process consists of a batch reactor followed by a purification unit. In this review, we show that other processes smartly combining the separation and the reaction unit operations within a single process can bring a competitive advantage in terms of yield, conversion and productivity. This represents an alternative approach to the development of new ad-hoc specific chemistries. In the following, in addition to provide an overview of processes described in the literature and discuss advantages and disadvantages of each, we propose innovative solutions which include continuous manufacturing technologies.

蛋白质PEG化在用于修饰和改善治疗性蛋白质的整个反应中起着重要作用。经典的生产过程包括间歇式反应器，然后是纯化单元。在这篇综述中，我们表明在单个过程中将分离和反应单元操作巧妙地结合在一起的其他过程可以在产量，转化率和生产率方面带来竞争优势。这代表了开发新的特定化学品的替代方法。在下文中，除了提供对文献中描述的过程的概述并讨论每种方法的优缺点之外，我们还提出了创新的解决方案，其中包括连续制造技术。