Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO₂) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility. The PAH-ACP released from loaded hmZrO₂ still retained the ability to infiltrate and mineralize collagen fibrils as well as exhibited osteoinductivity. A collagen scaffold blended with PAH-ACP@hmZrO₂ supplement and stem cells may be a promising tool for bone tissue engineering.

Statement of Significance

The advent of nanotechnology has catalyzed the development of bone tissue engineering strategies based on the combination of nanocarrier delivery systems and scaffolds, which provide distinct advantages, including the possibilities of sustained release and protection of the bioactive agents, site-specific pharmacological effects and reduction of side effects. Herein, hollow mesoporous zirconia (hmZrO₂) nanocapsules with pH-sensitive capacity were synthesized for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of ACP (PAH-ACP). The loaded nanocapsules show good biocompatibility and demonstrate bioactivities for collagen biomineralization and bone marrow stromal cells osteoinduction. Our results may offer a promising tool for designing bone tissue engineering “cocktail therapy” involving seeding scaffolds with biomineralization precursors loaded hmZrO₂ supplement and stem cells.

中文翻译：

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空心介孔氧化锆输送系统，用于生物矿化前体

基于纳米载体递送系统和支架的组合的策略为骨组织工程支架提供了多功能。氧化锆是一种通常在整形外科和牙科植入物中使用的生物相容性陶瓷，用于合成中空介孔纳米胶囊，以装载，储存和持续释放新型的多胺稳定的无定形磷酸钙（PAH-ACP）胶原蛋白矿化和骨骼的液态前体相。骨髓基质细胞骨诱导。装有仿生前体的中空氧化锆（hmZrO ₂）纳米胶囊具有pH敏感的释放能力和良好的生物相容性。从加载的hmZrO _2中释放出PAH-ACP仍然保留了渗透和矿化胶原蛋白原纤维的能力，以及表现出的骨诱导能力。与PAH-ACP @ hmZrO ₂补充剂和干细胞混合的胶原蛋白支架可能是用于骨组织工程的有前途的工具。

重要声明

纳米技术的出现促进了基于纳米载体递送系统和支架相结合的骨组织工程策略的发展，这些策略提供了独特的优势，包括持续释放和保护生物活性剂，特定部位的药理作用以及减少骨关节炎的可能性。副作用。在此，中空介孔氧化锆（hmZrO ₂）合成了具有pH敏感能力的纳米胶囊，用于装载，储存和持续释放新型多胺稳定的ACP液体前体相（PAH-ACP）。负载的纳米胶囊显示出良好的生物相容性，并显示出用于胶原生物矿化和骨髓基质细胞骨诱导的生物活性。我们的研究结果可能为设计骨组织工程“鸡尾酒疗法”提供有前途的工具，其中包括在支架上植入生物矿化前体，其中负载了hmZrO ₂补充剂和干细胞。