Organ specific uptake and depuration, and biological effects in Atlantic salmon (Salmo salar) exposed to 2, 4, 6-trinitrotoluene (TNT) were studied. Two experiments were conducted, the first using radiolabeled TNT (¹⁴C-TNT, 0.16 mg/L) to study uptake (48 h) and depuration (48 h), while the second experiment focused on physiological effects in fish exposed to increasing concentrations of unlabeled TNT (1 μg–1 mg/L) for 48 h. The uptake of ¹⁴C-TNT in the gills and most of the organs increased rapidly during the first 6 h of exposure (12 h in the brain) followed by a rapid decrease even though the fish were still exposed to TNT in the water. The radioactivity in the gall bladder reached a maximum after 55 h, 7 h after the transfer to the clean water. A high concentration of ¹⁴C-TNT in the gall bladder indicates that TNT is excreted through the gall bladder. Mortality (2 out of 14) was observed at a concentration of 1 mg/L, and the surviving fish had hemorrhages in the dorsal muscle tissue near the spine. Analysis of the physiological parameters in blood from the high exposure group revealed severe effects, with an increase in the levels of glucose, urea and HCO₃, and a decrease in hematocrit and the levels of Cl and hemoglobin. No effects on blood physiology were observed in fish exposed to the lower concentrations of TNT (1–100 μg/L). TNT and the metabolites 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) were found in the muscle tissue, whereas only 2-ADNT and 4-ADNT were found in the bile. The rapid excretion and estimated bioconcentration factors (range of 2–18 after 48 h in gills, blood, liver, kidney, muscle and brain) indicated a low potential for bioaccumulation of TNT.

研究了暴露于2，4，6-三硝基甲苯（TNT）的大西洋鲑（Salmo salar）中的器官特异性摄取和净化以及生物学效应。进行了两个实验，第一个实验是使用放射性标记的TNT（¹⁴ C-TNT，0.16 mg / L）研究摄取（48小时）和纯净（48小时），而第二个实验的重点是暴露于浓度不断增加的鱼类中的生理效应。 48小时未标记的TNT（1 μg–1 mg / L）。吸收¹⁴fish和大部分器官中的C-TNT在暴露的前6小时（大脑中为12小时）迅速增加，随后迅速降低，即使鱼仍在水中暴露于TNT中。转移到纯净水中后7小时（55小时），胆囊中的放射性达到最大值。胆囊中高浓度的¹⁴ C-TNT表示TNT通过胆囊排出。在1 mg / L的浓度下观察到死亡率（14个中的2个），存活的鱼在脊柱附近的背肌组织中有出血。高暴露人群血液中的生理参数分析显示出严重的影响，葡萄糖，尿素和HCO ₃含量增加，血细胞比容降低，Cl和血红蛋白水平降低。暴露于较低浓度的TNT（1–100μg/ L）的鱼类中未观察到对血液生理的影响。在肌肉组织中发现了TNT和代谢物2-氨基-4,6-二硝基甲苯（2-ADNT）和4-氨基-2,6-二硝基甲苯（4-ADNT），而只有2-ADNT和4-ADNT在胆汁中发现。快速排泄和估计的生物富集因子（48，血液，肝脏，肾脏，肌肉和大脑48小时后为2–18）表明TNT的生物蓄积潜力较低。