Abstract The total polyphenolic and flavonoid contents, as well as the antioxidant free radical scavenging activity (DPPH test) of different sieved powders of Hedera helix (L.) and Scrophularia nodosa (L.), were assessed. Polyphenolic compound content varied significantly between different particle sizes (20–50; 50–100; 100–180; 180–315; > 500 μm). The intermediate classes (between 100 μm and 315 μm) exhibited the highest total polyphenol content with 28.3 mg gallic acid eq./g dry matter (DM), total flavonoid content (8.6 mg catechin eq./g DM) and antioxidant activity (23.5 Trolox eq. mg/g DM). The lowest values were observed for the unsieved powder of Hedera helix (L.), and > 500 μm classes of Scrophularia nodosa (L.). Liquid chromatography coupled with UV detector and mass spectrometry (LC-PDA-ESI/MS) allowed identifying and quantifying polyphenols, flavonoids, iridoids, and triterpene saponins. These latter were exclusively identified by mass spectrometry; unusable signals being recorded by UV measurements. Results showed that most of the compounds presented maximal concentrations in intermediate particle sizes (100 μm–315 μm).

中文翻译：

---

Hedera helix ( L. ) 和 Scrophularia nodosa ( L. ) 的最佳多酚和三萜含量：粉末粒径的作用

摘要 评估了常春藤(L.)和玄参(L.)不同筛粉的总多酚和黄酮类含量，以及抗氧化自由基清除活性(DPPH测试)。多酚化合物含量在不同粒径（20–50；50–100；100–180；180–315；> 500 μm）之间变化很大。中间类别（100 微米和 315 微米之间）表现出最高的总多酚含量，28.3 毫克没食子酸当量/克干物质（DM）、总黄酮含量（8.6 毫克儿茶素当量/克 DM）和抗氧化活性（23.5 Trolox eq.mg/g DM)。观察到未筛分的常春藤粉末 (L.) 和 > 500 μm 等级的玄参 (L.) 的最低值。液相色谱结合紫外检测器和质谱 (LC-PDA-ESI/MS) 可以识别和定量多酚、黄酮类化合物、环烯醚萜类化合物和三萜皂苷。后者仅通过质谱法鉴定；UV 测量记录了不可用的信号。结果表明，大多数化合物在中等粒径 (100 μm–315 μm) 中呈现最大浓度。