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Characterization and assessment of two biocontrol bacteria against Pseudomonas syringae wilt in Solanum lycopersicum and its genetic responses
Microbiological Research ( IF 6.7 ) Pub Date : 2017-09-12 , DOI: 10.1016/j.micres.2017.09.003
Kaliannan Durairaj , Palanivel Velmurugan , Jung-Hee Park , Woo-Suk Chang , Yool-Jin Park , Palaninaicker Senthilkumar , Kyung-Min Choi , Jeong-Ho Lee , Byung-Taek Oh

Pseudomonas and Bacillus species are attractive due to their potential bio-control application against plant bacterial pathogens. Pseudomonas aeruginosa strain D4 and Bacillus stratosphericus strain FW3 were isolated from mine tailings in South Korea. In these potent bacterial strains, we observed improved antagonistic activity against Pseudomonas syringae DC3000. These strains produced biocatalysts for plant growth promotion, and in vivo examination of Solanum lycopersicum included analysis of disease severity, ion leakage, chlorophyll content, and H2O2 detection. In addition, regulation of the defense genes pathogen-related protein 1a (PR1a) and phenylalanine ammonia lyase (PAL) was compared with treated plants and untreated control plants. The results suggest that these two bacterial strains provide protection against plant pathogens via direct and indirect modes of action and could be used as a bio-control agent.



中文翻译:

两种抗番茄假单胞菌枯萎病的生防菌的鉴定,鉴定及其遗传响应

假单胞菌属芽孢杆菌属种由于其对植物细菌病原体的潜在生物防治应用而具有吸引力从韩国的尾矿中分离出铜绿假单胞菌D4株和平流芽孢杆菌FW3株。在这些强大的细菌菌株中,我们观察到针对丁香假单胞菌DC3000的拮抗活性得到了改善。这些菌株产生了促进植物生长的生物催化剂,对茄果茄的体内检查包括对疾病严重性,离子泄漏,叶绿​​素含量和H 2 O 2的分析。检测。另外,将防御基因病原体相关蛋白1a(PR1a)和苯丙氨酸氨裂合酶(PAL)的调节与处理过的植物和未处理过的对照植物进行了比较。结果表明,这两种细菌菌株通过直接和间接作用方式提供了针对植物病原体的保护作用,可以用作生物防治剂。

更新日期:2017-09-12
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