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SawR a new regulator controlling pyomelanin synthesis in Pseudomonas aeruginosa
Microbiological Research ( IF 6.7 ) Pub Date : 2017-10-13 , DOI: 10.1016/j.micres.2017.10.004
Yossi Ben-David , Elena Zlotnik , Itzhak Zander , Gal Yerushalmi , Sivan Shoshani , Ehud Banin

Surface Acoustic Waves (SAW) were previously shown to inhibit biofilm formation, increase bacterial susceptibility to antibiotic treatment and alter the transcription pattern of Pseudomonas aeruginosa. Here we characterize one gene, sawR (PA3133), that is highly overexpressed when P. aeruginosa is exposed to SAW. SawR is a putative transcription factor belonging to the TetR regulator family. When overexpressed sawR causes numerous phenotypes, including the accumulation of a brown pigment which we identified as pyomelanin. In this study we describe how sawR regulates pyomelanin synthesis. We show that sawR down-regulates the expression levels of hmgA and this causes the accumulation of homogentisic acid which in turn undergoes oxidation and polymerization to pyomelanin. Using bioinformatics, we were able to identify a specific amino acid, arginine 23, which is found within the sawR DNA binding domain and is crucial for its regulatory activity. Our results indicate that sawR does not affect any other genes in the phenylalanine/tyrosine metabolic pathway and its repressive ability on hmgA is not mediated by the hmgA repressor PA2010 (i.e. hmgR). Taken together, our results shed light on the regulatory cascade controlling pyomelanin synthesis and uncover yet another unknown regulator involved in its regulation.



中文翻译:

SawR是一种新的调节铜绿假单胞菌中脓疱素合成的调节剂

先前已显示表面声波(SAW)抑制生物膜形成,增加细菌对抗生素治疗的敏感性并改变铜绿假单胞菌的转录模式。在这里,我们描述了一个基因,sawR(PA3133),当铜绿假单胞菌暴露于SAW时该基因过度表达。SawR是属于TetR调节子家族的推定转录因子。过度表达时,sawR会导致许多表型,包括棕色色素的积聚,我们将其识别为pyomelanin。在这项研究中,我们描述了sawR如何调节pyomelanin的合成。我们发现,SAWR下调的表达水平HMGA这会导致高纯酸的积累,进而使高纯酸发生氧化和聚合,生成吡美拉宁。使用生物信息学,我们能够鉴定出一种特定的氨基酸,精氨酸23,其位于sawR DNA结合结构域内,对其调节活性至关重要。我们的结果表明,sawR不会影响苯丙氨酸/酪氨酸代谢途径中的任何其他基因,并且其对hmgA的抑制能力不受hmgA阻遏物PA2010(即hmgR)介导。综上所述,我们的结果揭示了控制品红素合成的调控级联反应,并揭示了另一个参与其调控的未知调控子。

更新日期:2017-10-13
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