Filamentous ascomycete Phomopsis sp. are common inhabitants of natural ecosystems and, as saprophytes, are largely responsible for the destructive decay of litterfall, promoting the carbon and nitrogen cycles. Phomopsis liquidambari B3 can establish mutualistic symbiosis with a broad spectrum of crop plants. Colonizing dynamics observations and a growth promotion assay of rice and Arabidopsis thaliana revealed that the B3 colonization strategy is host-adapted and resulted in different growth promotions influenced by N availability. However, the biochemical mechanisms and underlying genetics of the saprophyte transition to an endophyte are poorly understood. Here, the transcriptome features of generalist P. liquidambari and highlighted gene sets involved in the lifestyle transition from saprophytism to endophytism were reported. Most notable were genes for translation, ribosome biogenesis and MAPK signaling, several of which were only up-regulated in endophytic B3. Coordinated up-regulation of genes encoding enzymes involved in phenylalanine, tyrosine and tryptophan biosynthesis were preceded by secondary metabolite induction, which was encountered with host defense. Quantitative PCR validates the reliability of RNA-seq. Dissection at the molecular level facilitated a deeper understanding of P. liquidambari adaptation to hosts and the complex natural environment to play a role in sustainable agriculture and carbon and nitrogen cycles.

丝状子囊菌Phomopsis sp。是自然生态系统的常见居民，作为腐生植物，主要​​负责凋落物的破坏性腐烂，促进了碳和氮的循环。拟南芥B3可以与多种农作物建立共生共生关系。水稻和拟南芥的定殖动力学观察和生长促进试验表明，B3的定殖策略是适应宿主的，并受氮的有效利用而导致不同的生长促进。但是，对腐生植物过渡到内生菌的生化机制和潜在遗传学知之甚少。在这里，通才P的转录组特征。Liquidambari并且报道了涉及从腐生到内生的生活方式转变的重要基因组。最值得注意的是用于翻译，核糖体生物发生和MA​​PK信号传导的基因，其中一些仅在内生B3中被上调。编码与苯丙氨酸，酪氨酸和色氨酸生物合成有关的酶的基因的协调上调之前是次级代谢产物的诱导，这在宿主防御中遇到。定量PCR验证了RNA-seq的可靠性。在分子水平上进行解剖有助于更深入地了解液态拟杆菌对寄主的适应以及复杂的自然环境在可持续农业以及碳和氮循环中的作用。