Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 × 105 Da. The major sugars were l-fucose, l-arabinose, d-galactose, d-xylose, and d-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount of d-glucose, d-mannose, l-rhamnose, and d-glucuronic acid. Results indicated that the bioactive polysaccharides consisted of a (1,4)-Galp and (1,4)-GalAp back bone; (1,2)-Xyl and (1,2)-Rha might also comprise backbone or constitute side chain; linkage (1,5)-Ara and terminal fucosyl residues were also involved in the polysaccharides. Regarding bioactivity, removal of the terminal l-fucosyl residues reduced the TNF-α cytokine stimulating activity of the polysaccharides in a RAW 264.7 macrophage cell-line test, whereas NF-κB and TLR4 affected the polysaccharide-induced TNF-α production.

中文翻译：

---

姬松茸深层发酵含岩藻糖多糖的特性

岩藻糖是许多免疫细胞识别模式的重要残基之一。在这项研究中，我们从姬松茸的深层发酵中表征了具有生物活性的含岩藻糖的酸性多糖。我们通过基于细胞的活性指导策略获得多糖，并使用基于碳水化合物识别单克隆抗体的酶联免疫吸附试验 (ELISA) 以及甲基化和核磁共振分析来研究多糖的结构特征。多糖的 Mw 为 3.5 × 105 Da。主要糖为l-岩藻糖、l-阿拉伯糖、d-半乳糖、d-木糖和d-半乳糖醛酸，摩尔比分别为6.4、15.5、28.5、14.7和25.0%，还有少量d-葡萄糖， d-甘露糖、l-鼠李糖和d-葡萄糖醛酸。结果表明，生物活性多糖由（1，4)-Galp 和 (1,4)-GalAp 骨干；(1,2)-Xyl和(1,2)-Rha也可以包含主链或构成侧链；连接(1,5)-Ara 和末端岩藻糖基残基也参与多糖。关于生物活性，在 RAW 264.7 巨噬细胞系测试中，末端 l-岩藻糖基残基的去除降低了多糖的 TNF-α 细胞因子刺激活性，而 NF-κB 和 TLR4 影响了多糖诱导的 TNF-α 产生。