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NAD+ precursors promote the restoration of spermatogenesis in busulfan-treated mice through inhibiting Sirt2-regulated ferroptosis Theranostics (IF 12.4) Pub Date : 2024-4-15 Yan-Qin Feng, Xuan Liu, Ning Zuo, Mu-Bin Yu, Wen-Meng Bian, Bao-Quan Han, Zhong-Yi Sun, Massimo De Felici, Wei Shen, Lan Li
Rationale: In recent years, nicotinamide adenine dinucleotide (NAD+) precursors (Npre) have been widely employed to ameliorate female reproductive problems in both humans and animal models. However, whether and how Npre plays a role in the male reproductive disorder has not been fully clarified./nMethods: In the present study, a busulfan-induced non-obstructive azoospermic mouse model was used, and
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Size control of ropivacaine nano/micro-particles by soft-coating with peptide nanosheets for long-acting analgesia Theranostics (IF 12.4) Pub Date : 2024-4-15 Jing Liu, Weiwei Wu, Fei Peng, Deying Gong, Yi Kang, Yujun Zhang, Congyan Liu, Yuncheng Li, Guoyan Zhao, Feng Qiu, Wensheng Zhang
Rationale: To meet the need of long-acting analgesia in postoperative pain management, slow-releasing formulations of local anesthetics (LAs) have been extensively investigated. However, challenges still remain in obtaining such formulations in a facile and cost-effective way, and a mechanism for controlling the release rate to achieve an optimal duration is still missing./nMethods: In this study,
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Lipid nanoparticle encapsulated large peritoneal macrophages migrate to the lungs via the systemic circulation in a model of clodronate-mediated lung-resident macrophage depletion Theranostics (IF 12.4) Pub Date : 2024-4-8 Dhaval Oza, Fernando Ivich, Joshua Pace, Mikyung Yu, Mark Niedre, Mansoor Amiji
Rationale: A mature tissue resident macrophage (TRM) population residing in the peritoneal cavity has been known for its unique ability to migrate to peritoneally located injured tissues and impart wound healing properties. Here, we sought to expand on this unique ability of large peritoneal macrophages (LPMs) by investigating whether these GATA6+ LPMs could also intravasate into systemic circulation
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Mechanosensitive protein polycystin-1 promotes periosteal stem/progenitor cells osteochondral differentiation in fracture healing Theranostics (IF 12.4) Pub Date : 2024-4-8 Ran Liu, Yu-Rui Jiao, Mei Huang, Nan-Yu Zou, Chen He, Min Huang, Kai-Xuan Chen, Wen-Zhen He, Ling Liu, Yu-Chen Sun, Zhu-Ying Xia, L. Darryl Quarles, Hai-Lin Yang, Wei-Shan Wang, Zhou-Sheng Xiao, Xiang-Hang Luo, Chang-Jun Li
Background: Mechanical forces are indispensable for bone healing, disruption of which is recognized as a contributing cause to nonunion or delayed union. However, the underlying mechanism of mechanical regulation of fracture healing is elusive./nMethods: We used the lineage-tracing mouse model, conditional knockout depletion mouse model, hindlimb unloading model and single-cell RNA sequencing to analyze
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Exploring the role of combined external beam radiotherapy and targeted radioligand therapy with [177Lu]Lu-PSMA-617 for prostate cancer - from bench to bedside Theranostics (IF 12.4) Pub Date : 2024-4-8 Daria Arbuznikova, Aikaterini Klotsotyra, Lisa Uhlmann, Lisa-Charlotte Domogalla, Nils Steinacker, Michael Mix, Gabriele Niedermann, Simon K.B. Spohn, Martin T. Freitag, Anca L. Grosu, Philipp T. Meyer, Christian Gratzke, Matthias Eder, Constantinos Zamboglou, Ann-Christin Eder
Management of prostate cancer (PC) might be improved by combining external beam radiotherapy (EBRT) and prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT) with lutetium-177 (177Lu)-labeled PSMA inhibitors. We hypothesized a higher efficacy of the combination due to augmentation of the radiation dose to the tumor and interactions of EBRT with PSMA expression potentially increasing
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Hypofractionated radiotherapy combined with lenalidomide improves systemic antitumor activity in mouse solid tumor models Theranostics (IF 12.4) Pub Date : 2024-4-8 Kateryna Onyshchenko, Ren Luo, Xi Rao, Xuanwei Zhang, Simone Gaedicke, Anca-Ligia Grosu, Elke Firat, Gabriele Niedermann
Background: Hypofractionated radiotherapy (hRT) can induce a T cell-mediated abscopal effect on non-irradiated tumor lesions, especially in combination with immune checkpoint blockade (ICB). However, clinically, this effect is still rare, and ICB-mediated adverse events are common. Lenalidomide (lena) is an anti-angiogenic and immunomodulatory drug used in the treatment of hematologic malignancies
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Siglec-F+ neutrophils in the spleen induce immunosuppression following acute infection Theranostics (IF 12.4) Pub Date : 2024-4-8 Chaoxiong Liao, Shuhua Luo, Xiaolei Liu, Lina Zhang, Pengyun Xie, Wending Zhou, Yue Lu, Hanhui Zhong, Xuedi Zhang, Ziying Xiong, Xiao Huang, Guixi Mo, Daqing Ma, Jing Tang
Background: The mechanisms underlying the increased mortality of secondary infections during the immunosuppressive phase of sepsis remain elusive./nObjectives: We sought to investigate the role of Siglec-F+ neutrophils on splenic T lymphocytes in the immunosuppressed phase of sepsis and on secondary infection in PICS mice, and to elucidate the underlying mechanisms./nMethods: We established a mouse
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IL4 receptor targeting enables nab-paclitaxel to enhance reprogramming of M2-type macrophages into M1-like phenotype via ROS-HMGB1-TLR4 axis and inhibition of tumor growth and metastasis Theranostics (IF 12.4) Pub Date : 2024-4-8 Sri Murugan Poongkavithai Vadevoo, Yeoul Kang, Gowri Rangaswamy Gunassekaran, Seok-Min Lee, Min-Sung Park, Dong Gyun Jo, Sang-Kyun Kim, Ho Lee, Won Jong Kim, Byungheon Lee
Rationale: Nab-paclitaxel (Abx) is widely employed in malignant tumor therapy. In tumor cells and pro-tumoral M2-type macrophages, the IL4 receptor (IL4R) is upregulated. This study aimed to elucidate the selective delivery of Abx to M2-type macrophages by targeting IL4R and reprogramming them into an anti-tumoral M1-type./nMethods: Abx was conjugated with the IL4R-binding IL4RPep-1 peptide using click
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Nanotechnology in inflammation: cutting-edge advances in diagnostics, therapeutics and theranostics Theranostics (IF 12.4) Pub Date : 2024-4-8 Yuting Liu, Ziqi Lin, Yuting Wang, Liuhui Chen, Yuequan Wang, Cong Luo
Inflammatory dysregulation is intimately associated with the occurrence and progression of many life-threatening diseases. Accurate detection and timely therapeutic intervention on inflammatory dysregulation are crucial for the effective therapy of inflammation-associated diseases. However, the clinical outcomes of inflammation-involved disorders are still unsatisfactory. Therefore, there is an urgent
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Disturbed flow impairs MerTK-mediated efferocytosis in aortic endothelial cells during atherosclerosis Theranostics (IF 12.4) Pub Date : 2024-3-31 Jinzi Wu, Shijie Liu, Oishani Banerjee, Hang Shi, Bingzhong Xue, Zufeng Ding
Background: MER proto-oncogene tyrosine kinase (MerTK) is a key receptor for efferocytosis, a process for the clearance of apoptotic cells. MerTK is mainly expressed in macrophages and immature dendritic cells. There are very limited reports focused on MerTK biology in aortic endothelial cells (ECs). It remains unclear for the role of blood flow patterns in regulating MerTK-mediated efferocytosis in
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The HSP90 inhibitor HVH-2930 exhibits potent efficacy against trastuzumab-resistant HER2-positive breast cancer Theranostics (IF 12.4) Pub Date : 2024-3-31 Minsu Park, Eunsun Jung, Jung Min Park, Soeun Park, Dongmi Ko, Juyeon Seo, Seongjae Kim, Kee Dal Nam, Yong Koo Kang, Lee Farrand, Van-Hai Hoang, Cong-Truong Nguyen, Minh Thanh La, Gibeom Nam, Hyun-Ju Park, Jihyae Ann, Jeewoo Lee, Yoon-Jae Kim, Ji Young Kim, Jae Hong Seo
Rationale: Resistance to targeted therapies like trastuzumab remains a critical challenge for HER2-positive breast cancer patients. Despite the progress of several N-terminal HSP90 inhibitors in clinical trials, none have achieved approval for clinical use, primarily due to issues such as induction of the heat shock response (HSR), off-target effects, and unfavorable toxicity profiles. We sought to
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Theranostics - a sure cure for cancer after 100 years? Theranostics (IF 12.4) Pub Date : 2024-3-31 Yangmeihui Song, Jianhua Zou, E. Alejandro Castellanos, Naomi Matsuura, John A. Ronald, Adam Shuhendler, Wolfgang A Weber, Assaf A. Gilad, Cristina Müller, Timothy H. Witney, Xiaoyuan Chen
Cancer has remained a formidable challenge in medicine and has claimed an enormous number of lives worldwide. Theranostics, combining diagnostic methods with personalized therapeutic approaches, shows huge potential to advance the battle against cancer. This review aims to provide an overview of theranostics in oncology: exploring its history, current advances, challenges, and prospects. We present
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HDAC6-dependent deacetylation of NGF dictates its ubiquitination and maintains primordial follicle dormancy Theranostics (IF 12.4) Pub Date : 2024-3-25 Tuo Zhang, Yuntong Tong, Rengguang Zhu, Yaoyun Liang, Jixian Zhang, Chujiao Hu, Meina He, Zhu Hu, Zhiyi Shen, Jin Niu, Jingjing Zhang, Yuanyuan Yu, Bangming Jin, Shan Lei, Zhirui Zeng, Yingmin Wu, Zengmei Cheng, Ziwen Xiao, Bing Guo, Shuyun Zhao, Guoqiang Xu, Wei Pan, Tengxiang Chen
Rationale: Primordial follicles are limited in number and cannot be regenerated, dormant primordial follicles cannot be reversed once they enter a growth state. Therefore, the length of the female reproductive lifespan depends on the orderly progression and selective activation of primordial follicles, the mechanism of which remains unclear./nMethods: We used human ovarian cortical biopsy specimens
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Hepatic progenitor cell-originated ductular reaction facilitates liver fibrosis through activation of hedgehog signaling Theranostics (IF 12.4) Pub Date : 2024-3-25 Yonghong Hu, Xinyu Bao, Zheng Zhang, Long Chen, Yue Liang, Yan Qu, Qun Zhou, Xiaoxi Zhou, Jing Fang, Zhun Xiao, Yadong Fu, Hailin Yang, Wei Liu, Ying Lv, Hongyan Cao, Gaofeng Chen, Jian Ping, Hua Zhang, Yongping Mu, Chenghai Liu, Chao-Po Lin, Jian Wu, Ping Liu, Jiamei Chen
Background: It is poorly understood what cellular types participate in ductular reaction (DR) and whether DR facilitates recovery from injury or accelerates hepatic fibrosis. The aim of this study is to gain insights into the role of hepatic progenitor cell (HPC)-originated DR during fibrotic progression./nMethods: DR in liver specimens of PBC, chronic HBV infection (CHB) or NAFLD, and four rodent
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Transglutaminase 2 in diabetes mellitus: Unraveling its multifaceted role and therapeutic implications for vascular complications Theranostics (IF 12.4) Pub Date : 2024-3-25 Kwon-Soo Ha
Diabetes, a severe metabolic disease characterized by chronic hypoglycemia, poses debilitating and life-threatening risks of microvascular and macrovascular complications, including blindness, kidney failure, heart attacks, and limb amputation. Addressing these complications is paramount, urging the development of interventions targeting diabetes-associated vascular dysfunctions. To effectively combat
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Theranostics and artificial intelligence: new frontiers in personalized medicine Theranostics (IF 12.4) Pub Date : 2024-3-25 Gokce Belge Bilgin, Cem Bilgin, Brian J. Burkett, Jacob J. Orme, Daniel S. Childs, Matthew P. Thorpe, Thorvardur R Halfdanarson, Geoffrey B Johnson, Ayse Tuba Kendi, Oliver Sartor
The field of theranostics is rapidly advancing, driven by the goals of enhancing patient care. Recent breakthroughs in artificial intelligence (AI) and its innovative theranostic applications have marked a critical step forward in nuclear medicine, leading to a significant paradigm shift in precision oncology. For instance, AI-assisted tumor characterization, including automated image interpretation
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From molecules to medicine: thiol selective bioconjugation in synthesis of diagnostic and therapeutic radiopharmaceuticals Theranostics (IF 12.4) Pub Date : 2024-3-25 Iqra Bibi, Sajid Mushtaq, Kyo Chul Lee, Ji Ae Park, Jung Young Kim
Radiolabeling of biomolecules and cells with radiolabeled prosthetic groups has significant implications for nuclear medicine, imaging, and radiotherapy. Achieving site-specific and controlled incorporation of radiolabeled prostheses under mild reaction conditions is crucial for minimizing the impact on the bioactivity of the radiolabeled compounds. The targeting of natural and abundant amino acids
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Programming peptide-oligonucleotide nano-assembly for engineering of neoantigen vaccine with potent immunogenicity Theranostics (IF 12.4) Pub Date : 2024-3-17 Zhichu Xiang, Jianhua Lu, Shangrui Rao, Chenxing Fu, Yuying Yao, Yongdong Yi, Yang Ming, Weijian Sun, Weisheng Guo, Xiaoyuan Chen
Background: Neoantigen nanovaccine has been recognized as a promising treatment modality for personalized cancer immunotherapy. However, most current nanovaccines are carrier-dependent and the manufacturing process is complicated, resulting in potential safety concerns and suboptimal codelivery of neoantigens and adjuvants to antigen-presenting cells (APCs)./nMethods: Here we report a facile and general
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Role of the gut microbiota in tumorigenesis and treatment Theranostics (IF 12.4) Pub Date : 2024-3-17 Qingya Liu, Yun Yang, Meng Pan, Fan Yang, Yan Yu, Zhiyong Qian
The gut microbiota is a crucial component of the intricate microecosystem within the human body that engages in interactions with the host and influences various physiological processes and pathological conditions. In recent years, the association between dysbiosis of the gut microbiota and tumorigenesis has garnered increasing attention, as it is recognized as a hallmark of cancer within the scientific
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Dynamic pathological analysis reveals a protective role against skin fibrosis for TREM2-dependent macrophages Theranostics (IF 12.4) Pub Date : 2024-3-11 Yunsheng Liang, Yongfei Hu, Jun Zhang, Haosen Song, Xiaoqian Zhang, Yishan Chen, Yu Peng, Lihua Sun, Yuzhe Sun, Ruzeng Xue, Suyun Ji, Chuanwei Li, Zhili Rong, Bin Yang, Yingping Xu
Rationale: Systemic sclerosis (SSc) is a chronic and incurable autoimmune disease with high mortality rates, and skin fibrosis is one of distinguishing hallmarks in the pathogenesis. However, macrophage heterogeneity regulating skin fibrosis remain largely unknown./nMethods: We established mouse disease model and performed single-cell RNA-sequencing (scRNA-seq) to resolve the dynamic and heterogenous
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Myocardin reverses insulin resistance and ameliorates cardiomyopathy by increasing IRS-1 expression in a murine model of lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase V0d1 subunit Theranostics (IF 12.4) Pub Date : 2024-3-11 Wenlin Yuan, Hui Lin, Yuan Sun, Lihuan Liu, Meijuan Yan, Yujuan Song, Xiaofan Zhang, Xiangling Lu, Yipei Xu, Qiyue He, Kunfu Ouyang, Chenglin Zhang, Yong Pan, Yu Huang, Ying Li, Xifeng Lu, Jie Liu
Aim: Adipose tissue (AT) dysfunction that occurs in both obesity and lipodystrophy is associated with the development of cardiomyopathy. However, it is unclear how dysfunctional AT induces cardiomyopathy due to limited animal models available. We have identified vacuolar H+-ATPase subunit Vod1, encoded by Atp6v0d1, as a master regulator of adipogenesis, and adipose-specific deletion of Atp6v0d1 (Atp6v0d1AKO)
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Extracellular vesicles as carriers of mRNA: Opportunities and challenges in diagnosis and treatment Theranostics (IF 12.4) Pub Date : 2024-3-11 Robert D. Kirian, Darby Steinman, Christopher M. Jewell, Hannah C. Zierden
Extracellular vesicles (EVs) are produced by all cells in the body. These biological nanoparticles facilitate cellular communication through the transport of diverse cargoes, including small molecules, proteins, and nucleic acids. mRNA cargoes have gained particular interest given their role in the translation of functional proteins. As a biomarker platform, EVs can be found in nearly all biofluids—blood
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An advanced comprehensive muti-cell-type-specific model for predicting anti-PD-1 therapeutic effect in melanoma Theranostics (IF 12.4) Pub Date : 2024-3-3 Wei Sun, Yu Zhu, Zijian Zou, Lu Wang, Jingqin Zhong, Kangjie Shen, Xinyi Lin, Zixu Gao, Wanlin Liu, Yinlam Li, Yu Xu, Ming Ren, Tu Hu, Chuanyuan Wei, Jianying Gu, Yong Chen
Rationale: Immune checkpoint inhibitors targeting the programmed cell death (PD)-1/PD-L1 pathway have promise in patients with advanced melanoma. However, drug resistance usually results in limited patient benefits. Recent single-cell RNA sequencing studies have elucidated that MM patients display distinctive transcriptional features of tumor cells, immune cells and interstitial cells, including loss
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FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation Theranostics (IF 12.4) Pub Date : 2024-3-3 Xingyan Xu, Xuefen Zhuang, Haowei Yu, Ping Li, Xiaosa Li, Huiping Lin, Jian-peng Teoh, Yiwen Chen, Yuanlan Yang, Yang Cheng, Weiyu Chen, Xiaodong Fu
Background: The therapeutic benefits of targeting follicle-stimulating hormone (FSH) receptor in treatment of ovarian cancer are significant, whereas the role of FSH in ovarian cancer progresses and the underlying mechanism remains to be developed./nMethods: Tissue microarray of human ovarian cancer, tumor xenograft mouse model, and in vitro cell culture were used to investigate the role of FSH in
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Immunotherapeutic IL-6R and targeting the MCT-1/IL-6/CXCL7/PD-L1 circuit prevent relapse and metastasis of triple-negative breast cancer Theranostics (IF 12.4) Pub Date : 2024-3-3 Aushia Tanzih Al Haq, Pao-Pao Yang, Christopher Jin, Jou-Ho Shih, Li-Mei Chen, Hong-Yu Tseng, Yen-An Chen, Yueh-Shan Weng, Lu-Hai Wang, Michael P. Snyder, Hsin-Ling Hsu
Rationale: Multiple copies in T-cell malignancy 1 (MCT-1) is a prognostic biomarker for aggressive breast cancers. Overexpressed MCT-1 stimulates the IL-6/IL-6R/gp130/STAT3 axis, which promotes epithelial-to-mesenchymal transition and cancer stemness. Because cancer stemness largely contributes to the tumor metastasis and recurrence, we aimed to identify whether the blockade of MCT-1 and IL-6R can
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G protein subunit alpha i2's pivotal role in angiogenesis Theranostics (IF 12.4) Pub Date : 2024-3-3 Chao-wen Bai, Lu Lu, Jia-nan Zhang, Chengyu Zhou, Yi-chao Ni, Ke-ran Li, Jin Yao, Xiao-zhong Zhou, Chang-gong Lan, Cong Cao
Here we explored the potential role of Gαi2 (G protein subunit alpha i2) in endothelial cell function and angiogenesis./nMethods: Genetic methodologies such as shRNA, CRISPR/Cas9, dominant negative mutation, and overexpression were utilized to modify Gαi2 expression or regulate its function. Their effects on endothelial cell functions were assessed in vitro. In vivo, the endothelial-specific Gαi2 shRNA
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Intravital imaging of splenic classical monocytes modifying the hepatic CX3CR1+ cells motility to exacerbate liver fibrosis via spleen-liver axis Theranostics (IF 12.4) Pub Date : 2024-3-3 Chenlu Han, Yujie Zhai, Yuke Wang, Xuwen Peng, Xian Zhang, Bolei Dai, Yuehong Leng, Zhihong Zhang, Shuhong Qi
CX3CR1+ cells play a crucial role in liver fibrosis progression. However, changes in the migratory behavior and spatial distribution of spleen-derived and hepatic CX3CR1+ cells in the fibrotic liver as well as their influence on the liver fibrosis remain unclear./nMethods: The CX3CR1GFP/+ transgenic mice and CX3CR1-KikGR transgenic mice were used to establish the CCl4-induced liver fibrosis model.
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Engineered exosomes and composite biomaterials for tissue regeneration Theranostics (IF 12.4) Pub Date : 2024-3-3 Weikang Hu, Wang Wang, Zesheng Chen, Yun Chen, Zijian Wang
Exosomes, which are small vesicles enclosed by a lipid bilayer and released by many cell types, are widely dispersed and have garnered increased attention in the field of regenerative medicine due to their ability to serve as indicators of diseases and agents with therapeutic potential. Exosomes play a crucial role in mediating intercellular communication through the transfer of many biomolecules,
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Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases Theranostics (IF 12.4) Pub Date : 2024-2-25 Xingran Liu, Jing Yang, Yuxin Yan, Qingfeng Li, Ru-Lin Huang
Obesity-related metabolic diseases, including obesity, diabetes, hyperlipidemia, and non-alcoholic fatty liver diseases pose a significant threat to health. However, comprehensive pathogenesis exploration and effective therapy development are impeded by the limited availability of human models. Notably, advances in organoid technology enable the generation of adipose organoids that recapitulate structures
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Precision peptide theranostics: developing N- to C-terminus optimized theranostics targeting cholecystokinin-2 receptor Theranostics (IF 12.4) Pub Date : 2024-2-24 Marwa N. Rahimi, Alicia Corlett, Jessica Van Zuylekom, Marc Antoine Sani, Benjamin Blyth, Philip Thompson, Peter D. Roselt, Mohammad B. Haskali
Peptides are ideal for theranostic development as they afford rapid target accumulation, fast clearance from background tissue, and exhibit good tissue penetration. Previously, we developed a novel series of peptides that presented discreet folding propensity leading to an optimal candidate [68Ga]Ga-DOTA-GA1 ([D-Glu]6-Ala-Tyr-NMeGly-Trp-NMeNle-Asp-Nal-NH2) with 50 pM binding affinity against cholecystokinin-2
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[161Tb]Tb-PSMA-617 radioligand therapy in patients with mCRPC: preliminary dosimetry results and intra-individual head-to-head comparison to [177Lu]Lu-PSMA-617 Theranostics (IF 12.4) Pub Date : 2024-2-24 Andrea Schaefer-Schuler, Caroline Burgard, Arne Blickle, Stephan Maus, Christine Petrescu, Sven Petto, Mark Bartholomä, Tobias Stemler, Samer Ezziddin, Florian Rosar
Rationale: Evaluation of alternative radionuclides for use in prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT) is currently focusing on 161Tb, which may provide advantages by emitting additional Auger and conversion electrons. In this pilot study, we present preliminary dosimetry data for [161Tb]Tb-PSMA-617 RLT in a direct comparison with [177Lu]Lu-PSMA-617./nMethod: Six
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Dual regulation of NEMO by Nrf2 and miR-125a inhibits ferroptosis and protects liver from endoplasmic reticulum stress-induced injury Theranostics (IF 12.4) Pub Date : 2024-2-24 Jihoon Tak, Min Sung Joo, Yun Seok Kim, Hyun Woo Park, Chang Hoon Lee, Gil-Chun Park, Shin Hwang, Sang Geon Kim
Rationale: The surge of severe liver damage underscores the necessity for identifying new targets and therapeutic agents. Endoplasmic reticulum (ER) stress induces ferroptosis with Gα12 overexpression. NF-κB essential modulator (NEMO) is a regulator of inflammation and necroptosis. Nonetheless, the regulatory basis of NEMO de novo synthesis and its impact on hepatocyte ferroptosis need to be established
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Site-specific albumin tagging with NIR-II fluorogenic dye for high-performance and super-stable bioimaging Theranostics (IF 12.4) Pub Date : 2024-2-24 Ningning Zhu, Jiajun Xu, Qi Su, Tianyang Han, Ding Zhou, Yuewei Zhang, Shoujun Zhu
Synthetic near-infrared-II (NIR-II) dyes are promising for deep tissue imaging, yet they are generally difficult to target a given biomolecule with high specificity. Furthermore, the interaction mechanism between albumin and cyanine molecules, which is usually regarded as uncertain "complexes" such as crosslinked nanoparticles, remains poorly understood./nMethods: Here, we propose a new class of NIR-II
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Cancer-associated fibroblast spatial heterogeneity and EMILIN1 expression in the tumor microenvironment modulate TGF-β activity and CD8+ T-cell infiltration in breast cancer Theranostics (IF 12.4) Pub Date : 2024-2-24 Chikako Kanno Honda, Sasagu Kurozumi, Takaaki Fujii, Didier Pourquier, Lakhdar Khellaf, Florence Boissiere, Jun Horiguchi, Tetsunari Oyama, Ken Shirabe, Jacques Colinge, Takehiko Yokobori, Andrei Turtoi
Rationale: The tumor microenvironment (TME) and its multifaceted interactions with cancer cells are major targets for cancer treatment. Single-cell technologies have brought major insights into the TME, but the resulting complexity often precludes conclusions on function./nMethods: We combined single-cell RNA sequencing and spatial transcriptomic data to explore the relationship between different cancer-associated
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Fibroblasts facilitate lymphatic vessel formation in transplanted heart Theranostics (IF 12.4) Pub Date : 2024-2-24 Hui Gong, Ting Wang, Xiaotong Sun, Yuesheng Zhang, Yichao Qiu, Wenlong Sun, Yue Zhang, Peng Teng, Yanhua Hu, Xiaosheng Hu, Liang Ma, Qingbo Xu, Haige Zhao
Rationale: Lymphangiogenesis plays a critical role in the transplanted heart. The remodeling of lymphatics in the transplanted heart and the source of newly formed lymphatic vessels are still controversial, especially the mechanism of lymphangiogenesis remains limited./nMethods: Heart transplantation was performed among BALB/c, C57BL/6J, Cag-Cre, Lyve1-CreERT2;Rosa26-tdTomato and Postn(2A-CreERT2-
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Neutrophil extracellular trap-mediated impairment of meningeal lymphatic drainage exacerbates secondary hydrocephalus after intraventricular hemorrhage Theranostics (IF 12.4) Pub Date : 2024-2-24 Qiang Zhang, Yujie Chen, Yingpei Li, Zhou Feng, Liang Liang, Xiaoke Hao, Wenbo Kang, Zhaoqi Zhang, Xuyang Zhang, Rong Hu, Hua Feng, Zhi Chen
Rationale: Hydrocephalus is a substantial complication after intracerebral hemorrhage (ICH) or intraventricular hemorrhage (IVH) that leads to impaired cerebrospinal fluid (CSF) circulation. Recently, brain meningeal lymphatic vessels (mLVs) were shown to serve as critical drainage pathways for CSF. Our previous studies indicated that the degradation of neutrophil extracellular traps (NETs) after ICH/IVH
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Multifunctional Sr/Se co-doped ZIF-8 nanozyme for chemo/chemodynamic synergistic tumor therapy via apoptosis and ferroptosis Theranostics (IF 12.4) Pub Date : 2024-2-24 Aimin Wu, Ming Han, Zihan Ni, Haoran Li, Yinyin Chen, Zhouping Yang, Yumei Feng, Zufeng He, Hua Zhen, Xianxiang Wang
Rationale: Cancer continues to be a significant public health issue. Traditional treatments such as surgery, radiotherapy, and chemotherapy often fall short because of intrinsic issues such as lack of specificity and poor drug delivery, leading to insufficient drug concentration at the tumor site and/or potential side effects. Consequently, improving the delivery of conventional chemotherapy drugs
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H-ferritin-nanocaged gadolinium nanoparticles for ultra-sensitive MR molecular imaging Theranostics (IF 12.4) Pub Date : 2024-2-24 Jianlin Zhang, Chang Yuan, Lingfei Kong, Feiyan Zhu, Wanzhong Yuan, Junying Zhang, Juanji Hong, Fang Deng, Qi Chen, Chen Chen, Tao Wang, Zhentao Zuo, Minmin Liang
Rationale: Magnetic resonance imaging (MRI) is a powerful diagnostic technology by providing high-resolution imaging. Although MRI is sufficiently valued in its resolving morphology, it has poor sensitivity for tracking biomarkers. Therefore, contrast agents are often used to improve MRI diagnostic sensitivity. However, the clinically used Gd chelates are limited in improving MRI sensitivity owing
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Depletion of ApoA5 aggravates spontaneous and diet-induced nonalcoholic fatty liver disease by reducing hepatic NR1D1 in hamsters Theranostics (IF 12.4) Pub Date : 2024-2-24 Jiabao Guo, Guolin Miao, Wenxi Zhang, Haozhe Shi, Pingping Lai, Yitong Xu, Lianxin Zhang, Gonglie Chen, Yufei Han, Ying Zhao, Geroge Liu, Ling Zhang, Yuhui Wang, Wei Huang, Xunde Xian
Background: ApoA5 mainly synthesized and secreted by liver is a key modulator of lipoprotein lipase (LPL) activity and triglyceride-rich lipoproteins (TRLs). Although the role of ApoA5 in extrahepatic triglyceride (TG) metabolism in circulation has been well documented, the relationship between ApoA5 and nonalcoholic fatty liver disease (NAFLD) remains incompletely understood and the underlying molecular
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NPC1 is required for postnatal islet β cell differentiation by maintaining mitochondria turnover Theranostics (IF 12.4) Pub Date : 2024-2-24 Bei Liu, Duanyi Hua, Linyan Shen, Tingting Li, Zheying Tao, Chenyang Fu, Zhongzheng Tang, Jie Yang, Li Zhang, Aifang Nie, Yiran Jiang, Jiqiu Wang, Yang Li, Yanyun Gu, Guang Ning
Rationale: NPC1 is a protein localized on the lysosome membrane regulating intracellular cholesterol transportation and maintaining normal lysosome function. GWAS studies have found that NPC1 variants in T2D was a pancreatic islet expression quantitative trait locus, suggesting a potential role of NPC1 in T2D islet pathophysiology./nMethods: Two-week-old Npc1-/- mice and wild type littermates were
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Cancer diagnosis using label-free SERS-based exosome analysis Theranostics (IF 12.4) Pub Date : 2024-2-24 Yajuan Liu, Mei Li, Haisha Liu, Chao Kang, Cheng Wang
Exosomes, carrying distinctive biomolecules reflective of their parent cell's status and origin, show promise as liquid biopsy biomarkers for cancer diagnosis. However, their clinical translation remains challenging due to their relatively low concentration in body fluids. Surface-Enhanced Raman spectroscopy (SERS) has recently gained significant attention as a label-free and sensitive technique for
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Highly oriented hydrogels for tissue regeneration: design strategies, cellular mechanisms, and biomedical applications Theranostics (IF 12.4) Pub Date : 2024-2-24 Jiuping Wu, Zhihe Yun, Wenlong Song, Tao Yu, Wu Xue, Qinyi Liu, Xinzhi Sun
Many human tissues exhibit a highly oriented architecture that confers them with distinct mechanical properties, enabling adaptation to diverse and challenging environments. Hydrogels, with their water-rich "soft and wet" structure, have emerged as promising biomimetic materials in tissue engineering for repairing and replacing damaged tissues and organs. Highly oriented hydrogels can especially emulate
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In vivo high-speed microscopy of microbubbles in the chorioallantoic membrane model Theranostics (IF 12.4) Pub Date : 2024-2-17 Rojin Anbarafshan, Carly Pellow, Kevin Kiezun, Hon Leong, David E. Goertz
Rationale: The acoustic stimulation of microbubbles within microvessels can elicit a spectrum of therapeutically relevant bioeffects from permeabilization to perfusion shutdown. These bioeffects ultimately arise from complex interactions between microbubbles and microvascular walls, though such interactions are poorly understood particularly at high pressure, due to a paucity of direct in vivo observations
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BitterDB database analysis plus cell stiffness screening identify flufenamic acid as the most potent TAS2R14-based relaxant of airway smooth muscle cells for therapeutic bronchodilation Theranostics (IF 12.4) Pub Date : 2024-2-17 Kai Ni, Bo Che, Rong Gu, Chunhong Wang, Hongyang Xu, Huiduo Li, Shiyan Cen, Mingzhi Luo, Linhong Deng
Rationale: Bitter taste receptors (TAS2Rs) are abundantly expressed in airway smooth muscle cells (ASMCs), which have been recognized as promising targets for bitter agonists to initiate relaxation and thereby prevent excessive airway constriction as the main characteristic of asthma. However, due to the current lack of tested safe and potent agonists functioning at low effective concentrations, there
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Mrc1+ macrophage-derived IGF1 mitigates crystal nephropathy by promoting renal tubule cell proliferation via the AKT/Rb signaling pathway Theranostics (IF 12.4) Pub Date : 2024-2-17 Linxi Huang, Wei Chen, Zhuojing Tan, Yunxiao Huang, Xinji Gu, Lantian Liu, Hongxia Zhang, Yihan Shi, Jiarong Ding, Chengjian Zheng, Zhiyong Guo, Bing Yu
Rationale: The present understanding of the cellular characteristics and communications in crystal nephropathy is limited. Here, molecular and cellular studies combined with single-cell RNA sequencing (scRNA-seq) were performed to investigate the changes in cell components and their interactions in glyoxylate-induced crystallized kidneys to provide promising treatments for crystal nephropathy./nMethods:
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Activatable prodrug for controlled release of an antimicrobial peptide via the proteases overexpressed in Candida albicans and Porphyromonas gingivalis Theranostics (IF 12.4) Pub Date : 2024-2-17 Lubna Amer, Maurice Retout, Jesse V. Jokerst
Candida albicans and Porphyromonas gingivalis are prevalent in the subgingival area where the frequency of fungal colonization increases with periodontal disease. Candida's transition to a pathogenic state and its interaction with P. gingivalis exacerbate periodontal disease severity. However, current treatments for these infections differ, and combined therapy remains unexplored. This work is based
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Terbium radionuclides for theranostic applications in nuclear medicine: from atom to bedside Theranostics (IF 12.4) Pub Date : 2024-2-17 Camille Van Laere, Michel Koole, Christophe M. Deroose, Michiel Van de Voorde, Kristof Baete, Thomas E. Cocolios, Charlotte Duchemin, Maarten Ooms, Frederik Cleeren
Terbium features four clinically interesting radionuclides for application in nuclear medicine: terbium-149, terbium-152, terbium-155, and terbium-161. Their identical chemical properties enable the synthesis of radiopharmaceuticals with the same pharmacokinetic character, while their distinctive decay characteristics make them valuable for both imaging and therapeutic applications. In particular,
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Focused ultrasound ablation of melanoma with boiling histotripsy yields abscopal tumor control and antigen-dependent dendritic cell activation Theranostics (IF 12.4) Pub Date : 2024-2-11 Eric A. Thim, Lydia E. Kitelinger, Fátima Rivera-Escalera, Alexander S. Mathew, Michael R. Elliott, Timothy N. J. Bullock, Richard J. Price
Background: Boiling histotripsy (BH), a mechanical focused ultrasound ablation strategy, can elicit intriguing signatures of anti-tumor immunity. However, the influence of BH on dendritic cell function is unknown, compromising our ability to optimally combine BH with immunotherapies to control metastatic disease./nMethods: BH was applied using a sparse scan (1 mm spacing between sonications) protocol
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Integrated electronic/fluidic microneedle system for glucose sensing and insulin delivery Theranostics (IF 12.4) Pub Date : 2024-2-11 Xinshuo Huang, Baoming Liang, Shuang Huang, Zhengjie Liu, Chuanjie Yao, Jingbo Yang, Shantao Zheng, Feifei Wu, Wan Yue, Ji Wang, Huijiuan Chen, Xi Xie
Background: Precise and dynamic blood glucose regulation is paramount for both diagnosing and managing diabetes. Continuous glucose monitoring (CGM) coupled with insulin pumps forms an artificial pancreas, enabling closed-loop control of blood glucose levels. Indeed, this integration necessitates advanced micro-nano fabrication techniques to miniaturize and combine sensing and delivery modules on a
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Cancer-associated fibroblasts reprogram cysteine metabolism to increase tumor resistance to ferroptosis in pancreatic cancer Theranostics (IF 12.4) Pub Date : 2024-2-11 Yuchao Zhu, Shuai Fang, Bolin Fan, Kaiwei Xu, Liu Xu, Linwei Wang, Lubin Zhu, Chunqu Chen, Ruoyu Wu, Jiajing Ni, Jianhua Wang
Background: Pancreatic ductal adenocarcinoma (PDAC) is an insidious, rapidly progressing malignancy of the gastrointestinal tract. Due to its dense fibrous stroma and complex tumor microenvironment, neither of which is sensitive to radiotherapy, pancreatic adenocarcinoma is one of the malignancies with the poorest prognosis. Therefore, detailed elucidation of the inhibitory microenvironment of PDAC
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Forskolin-driven conversion of human somatic cells into induced neurons through regulation of the cAMP-CREB1-JNK signaling Theranostics (IF 12.4) Pub Date : 2024-2-11 Guodong Wang, Dandan Zhang, Liangshan Qin, Quanhui Liu, Wenkui Tang, Mingxing Liu, Fan Xu, Fen Tang, Leping Cheng, Huiming Mo, Xiang Yuan, Zhiqiang Wang, Ben Huang
Human somatic cells can be reprogrammed into neuron cell fate through regulation of a single transcription factor or application of small molecule cocktails./nMethods: Here, we report that forskolin efficiently induces the conversion of human somatic cells into induced neurons (FiNs)./nResults: A large population of neuron-like phenotype cells was observed as early as 24-36 h post-induction. There
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The DNA-dependent protein kinase catalytic subunit exacerbates endotoxemia-induced myocardial microvascular injury by disrupting the MOTS-c/JNK pathway and inducing profilin-mediated lamellipodia degradation Theranostics (IF 12.4) Pub Date : 2024-2-4 Rongjun Zou, Wanting Shi, Xing Chang, Miao Zhang, Songtao Tan, Ruibing Li, Hao Zhou, Yukun Li, Ge Wang, Weihui Lv, Xiaoping Fan
Rationale: The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) promotes pathological mitochondrial fission during septic acute kidney injury. The mitochondrial open reading frame of the 12S rRNA type-c (MOTS-c) is a mitochondria-derived peptide that exhibits anti-inflammatory properties during cardiovascular illnesses. We explored whether endotoxemia-induced myocardial microvascular injury
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MAGL protects against renal fibrosis through inhibiting tubular cell lipotoxicity Theranostics (IF 12.4) Pub Date : 2024-2-4 Shan Zhou, Xian Ling, Jielin Zhu, Ye Liang, Qijian Feng, Chao Xie, Jiemei Li, Qiyan Chen, Shuangqin Chen, Jinhua Miao, Mengyao Zhang, Zhiru Li, Weiwei Shen, Xiaolong Li, Qinyu Wu, Xiaoxu Wang, Ruiyuan Liu, Cheng Wang, Fan Fan Hou, Yaozhong Kong, Youhua Liu, Lili Zhou
Rationale: Renal fibrosis, with no therapeutic approaches, is a common pathological feature in various chronic kidney diseases (CKD). Tubular cell injury plays a pivotal role in renal fibrosis. Commonly, injured tubular cells exhibit significant lipid accumulation. However, the underlying mechanisms remain poorly understood./nMethods: 2-arachidonoylglycerol (2-AG) levels in CKD patients and CKD model
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Global metabolomics revealed deviations from the metabolic aging clock in colorectal cancer patients Theranostics (IF 12.4) Pub Date : 2024-2-4 Long Zhang, Shaobo Mo, Xiurui Zhu, C. James Chou, Bo Jin, Zhi Han, James Schilling, Weili Liao, Sheeno Thyparambil, Ruben Y. Luo, John C. Whitin, Lu Tian, Seema Nagpal, Scott R. Ceresnak, Harvey J. Cohen, Doff B. McElhinney, Karl G. Sylvester, Yangming Gong, Chen Fu, Xuefeng B. Ling, Junjie Peng
Background: Markers of aging hold promise in the context of colorectal cancer (CRC) care. Utilizing high-resolution metabolomic profiling, we can unveil distinctive age-related patterns that have the potential to predict early CRC development. Our study aims to unearth a panel of aging markers and delve into the metabolomic alterations associated with aging and CRC./nMethods: We assembled a serum cohort
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20-HETE mediated TRPV1 activation drives allokinesis via MrgprA3+ neurons in chronic dermatitis Theranostics (IF 12.4) Pub Date : 2024-2-4 Guang Yu, Pei Liu, Xiaobao Huang, Mingxin Qi, Xue Li, Weimeng Feng, Erxin Shang, Yuan Zhou, Changming Wang, Yan Yang, Chan Zhu, Fang Wang, Zongxiang Tang, Jinao Duan
Rationale: Noxious stimuli are often perceived as itchy in patients with chronic dermatitis (CD); however, itch and pain mechanisms of CD are not known./nMethods: TRPV1 involvement in CD was analyzed using a SADBE induced CD-like mouse model, and several loss- and gain-of-function mouse models. Trigeminal TRPV1 channel and MrgprA3+ neuron functions were analyzed by calcium imaging and whole-cell patch-clamp
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Emerging role of extracellular vesicles in diabetic retinopathy Theranostics (IF 12.4) Pub Date : 2024-2-4 Junyan Zhu, Jin Huang, Yaoxiang Sun, Wenrong Xu, Hui Qian
Diabetic retinopathy (DR), a complex complication of diabetes mellitus (DM), is a leading cause of adult blindness. Hyperglycemia triggers DR, resulting in microvascular damage, glial apoptosis, and neuronal degeneration. Inflammation and oxidative stress play crucial roles during this process. Current clinical treatments for DR primarily target the advanced retinal disorder but offer limited benefits
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Development of CD46 targeted alpha theranostics in prostate cancer using 134Ce/225Ac-Macropa-PEG4-YS5 Theranostics (IF 12.4) Pub Date : 2024-1-27 Kondapa Naidu Bobba, Anil P. Bidkar, Anju Wadhwa, Niranjan Meher, Suchi Drona, Alexandre M. Sorlin, Scott Bidlingmaier, Li Zhang, David M. Wilson, Emily Chan, Nancy Y. Greenland, Rahul Aggarwal, Henry F. VanBrocklin, Jiang He, Jonathan Chou, Youngho Seo, Bin Liu, Robert R. Flavell
Rationale: 225Ac, a long-lived α-emitter with a half-life of 9.92 days, has garnered significant attention as a therapeutic radionuclide when coupled with monoclonal antibodies and other targeting vectors. Nevertheless, its clinical utility has been hampered by potential off-target toxicity, a lack of optimized chelators for 225Ac, and limitations in radiolabeling methods. In a prior study evaluating
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High-content stimulated Raman histology of human breast cancer Theranostics (IF 12.4) Pub Date : 2024-1-27 Hongli Ni, Chinmayee Prabhu Dessai, Haonan Lin, Wei Wang, Shaoxiong Chen, Yuhao Yuan, Xiaowei Ge, Jianpeng Ao, Nolan Vild, Ji-Xin Cheng
Histological examination is crucial for cancer diagnosis, however, the labor-intensive sample preparation involved in the histology impedes the speed of diagnosis. Recently developed two-color stimulated Raman histology could bypass the complex tissue processing to generates result close to hematoxylin and eosin staining, which is one of the golden standards in cancer histology. Yet, the underlying
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Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Theranostics (IF 12.4) Pub Date : 2024-1-27 Meng Gao, Tuo Zhang, Tengxiang Chen, Ziqi Chen, Zijian Zhu, Yang Wen, Shaogang Qin, Yibing Bao, Ting Zhao, Hengxing Li, Longping Liu, Ming Hao, Jianbin Wang, Fengchao Wang, Haibin Wang, Bo Zhou, Hua Zhang, Guoliang Xia, Chao Wang
Rationale: Premature ovarian insufficiency (POI) is an accelerated reduction in ovarian function inducing infertility. Folliculogenesis defects have been reported to trigger POI as a consequence of ovulation failure. However, the underlying mechanisms remain unclear due to the genetic complexity and heterogeneity of POI./nMethods: We used whole genome sequencing (WGS), conditional knockout mouse models
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Tripeptidyl peptidase II coordinates the homeostasis of calcium and lipids in the central nervous system and its depletion causes presenile dementia in female mice through calcium/lipid dyshomeostasis-induced autophagic degradation of CYP19A1 Theranostics (IF 12.4) Pub Date : 2024-1-27 Jin Zhao, Chengtong He, Xueyu Fan, Lin Wang, Liao Zhao, Hui Liu, Wujun Shen, Sanwei Jiang, Kaixuan Pei, Jingjing Gao, Yawei Qi, Yang Liu, Junqiang Zhao, Ruiling Zhang, Chengbiao Lu, Jia Tong, Jisen Huai
Rationale: Tripeptidyl peptidase II (TPP2) has been proven to be related to human immune and neurological diseases. It is generally considered as a cytosolic protein which forms the largest known protease complex in eukaryotic cells to operate mostly downstream of proteasomes for degradation of longer peptides. However, this canonical function of TPP2 cannot explain its role in a wide variety of biological