New sandwich hybridization assay (SHA) probes for detecting Pseudo-nitzschia species (P. arenysensis, P. fraudulenta, P. hasleana, P. pungens) are presented, along with updated cross-reactivity information on historical probes (SHA and FISH; fluorescence in situ hybridization) targeting P. australis and P. multiseries. Pseudo-nitzschia species are a cosmopolitan group of diatoms that produce varying levels of domoic acid (DA), a neurotoxin that can accumulate in finfish and shellfish and transfer throughout the food web. Consumption of infected food sources can lead to illness in humans (amnesic shellfish poisoning; ASP) and marine wildlife (domoic acid poisoning; DAP). The threat of human illness, along with economic loss from fishery closures has resulted in the implementation of monitoring protocols and intensive ecological studies. SHA probes have been instrumental in some of these efforts, as the technique performs well in complex heterogeneous sample matrices and has been adapted to benchtop and deployable (Environmental Sample Processor) platforms. The expanded probe set will enhance future efforts towards understanding spatial, temporal and successional patterns in species during bloom and non-bloom periods.

新夹心杂交测定（SHA），用于检测的探针拟菱形藻物种（P. arenysensis，P. fraudulenta，P. hasleana，P. pungens）被呈现，与历史上的探针（SHA和FISH更新交叉反应性信息一起;荧光原位杂交），针对澳大利亚假单胞菌和多系列假单胞菌。假性尼兹卡物种是世界性的硅藻群体，它们产生不同水平的海藻酸（DA），这是一种神经毒素，可以在鳍鱼和贝类中积累，并在整个食物网中转移。食用受感染的食物会导致人类疾病（遗忘性贝类中毒； ASP）和海洋野生动植物（十二酸中毒； DAP）。人类疾病的威胁，以及因关闭渔业而造成的经济损失，导致实施了监测方案和深入的生态研究。SHA探针在这些工作中发挥了重要作用，因为该技术在复杂的异构样本矩阵中表现良好，并且已适应于台式和可部署（环境样本处理器）平台。扩展的探针集将加强未来在理解空间，