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Assessing the use of artificial substrates to monitor Gambierdiscus populations in the Florida Keys
Harmful Algae ( IF 6.6 ) Pub Date : 2017-07-29 , DOI: 10.1016/j.hal.2017.07.007
Michael L. Parsons , Ashley L. Brandt , Amanda Ellsworth , Alex K. Leynse , Lacey K. Rains , Donald M. Anderson

Four distinct coastal locations were sampled on a monthly basis near Long Key (Florida Keys, USA) over a 13-month period to study Gambierdiscus population dynamics on different substrates, including four macrophyte species (Dictyota spp., Halimeda spp., Laurencia spp., and Thalassia testudinum) and three artificial substrates (polyvinyl chloride (PVC) tiles, burlap, and fiberglass window screen). Cell densities of Gambierdiscus were generally lower on Dictyota versus Halimeda and Laurencia. Cell densities of Gambierdiscus were significantly correlated among macrophyte hosts in 54% of the comparisons, and between macrophyte hosts and artificial substrates in 72% of the comparisons. Predictive slopes determined from regression analyses between cell densities on artificial substrates and macrophyte hosts indicated that, on an areal basis, fewer cells were present on macrophytes versus artificial substrates (cells cm−2) and that slope variation (error) among the different macrophytes and sites ranged from 5% to 200%, averaging 61% overall. As the data required log-transformation prior to analyses, this level of error translates into two-orders of magnitude in range of estimation of the overall average abundance of Gambierdiscus cells on macrophytes (135 cells g−1 wet weight); 20–2690 cells g−1 ww. The lack of consistent correlation among Gambierdiscus cell densities on macrophytes versus artificial substrates, coupled with the high level of error associated with the predictive slope estimations, indicates that extreme caution should be taken when interpreting the data garnered from artificial substrate deployments, and that such deployments should be thoroughly vetted prior to routine use for monitoring purposes.



中文翻译:

评估使用人造基质监测佛罗里达群岛的冈比亚鞭毛虫种群

在13个月内每月在Long Key(美国佛罗里达州的Keys)附近每月采样四个不同的沿海地区,以研究Gambierdiscus在不同底物上的种群动态,包括四种大型植物物种(Dictyota spp。,Halimeda spp。,Laurencia spp。 ,以及Thalassia testudinum)和三种人造基材(聚氯乙烯(PVC)瓷砖,粗麻布和玻璃纤维玻璃窗)。的细胞密度Gambierdiscus普遍较低上Dictyota仙掌藻凹顶。冈比亚菌的细胞密度54%的比较中大型植物宿主之间的显着相关,72%的比较中大型植物宿主与人工底物之间的相关性显着。通过对人造基质和大型植物宿主细胞密度之间的回归分析确定的预测斜率表明,按面积计算,大型植物中的细胞数量少于人工基质(细胞cm -2),并且不同大型植物和植物之间的斜率变化(误差)。网站的比例从5%到200%不等,总体平均为61%。作为数据来分析所要求数转化前,该电平误差的转换成大小在整体平均丰度的估计的范围内的两个订单Gambierdiscus上大型植物细胞(135个细胞克-1湿重); 20–2690格g -1 ww。大型植物与人工基质上的冈比亚细菌细胞密度之间缺乏一致的相关性,再加上与预测斜率估计值相关的高水平误差,这表明在解释从人工基质部署获得的数据时应格外谨慎,并且此类部署在进行常规监控之前,应彻底审查。

更新日期:2017-07-29
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