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Influence of storage temperature/time and atmosphere on survival and thermal inactivation of Escherichia coli ATCC 25922 inoculated to almond powder
Food Control ( IF 6 ) Pub Date : 2018-04-01 , DOI: 10.1016/j.foodcont.2017.11.029
Teng Cheng , Shaojin Wang

Abstract The purposes of this study were to explore combined effects of storage temperature, time and atmosphere on survival and thermal inactivation of pathogenic Salmonella Enteritidis PT 30 with nonpathogenic E. coli ATCC 25922 as a surrogate inoculated to low-moisture almond powder. A thermal death time test heating block system (TDT-HBS) was used to rapidly determine thermal inactivation of E. coli ATCC 25922. Surviving populations and D75-values of E. coli ATCC 25922 in the almond powder with 6.0% w.b. were determined monthly for 12-month storage under three packaging atmosphere conditions at −20, 4, and 24 °C. The results showed that survival and thermal inactivation curves of E. coli ATCC 25922 fitted well to both Weibull and first-order-kinetic models, respectively. The effects of storage atmosphere on reducing surviving populations and D75-values of E. coli ATCC 25922 became more remarkable as the storage temperature increased. The reductions of 2.55 log CFU/g for surviving populations and 31.2% for D75-values were observed after 12-months storage at 24 °C under modified atmosphere packaging (MAP), and holding for 50.4 min at 75 °C could reach at least 4-log reductions of E. coli ATCC 25922, which are required by the USDA for safe exportation. Storage under MAP at ambient temperature may hold potential to inhibit surviving populations and reduce thermal resistance of S. Enteritidis PT 30 in low-moisture almond powder.

中文翻译:

储存温度/时间和气氛对接种杏仁粉的大肠杆菌ATCC 25922存活和热灭活的影响

摘要 本研究的目的是探讨储存温度、时间和气氛对致病性肠炎沙门氏菌 PT 30 存活和热灭活的综合影响,并以非致病性大肠杆菌 ATCC 25922 作为替代品接种到低水分杏仁粉中。使用热死亡时间测试加热块系统 (TDT-HBS) 快速测定大肠杆菌 ATCC 25922 的热灭活情况。每月测定 6.0% wb 杏仁粉中大肠杆菌 ATCC 25922 的存活种群和 D75 值在 -20、4 和 24 °C 的三种包装气氛条件下储存 12 个月。结果表明,大肠杆菌 ATCC 25922 的存活曲线和热灭活曲线分别与 Weibull 和一级动力学模型拟合良好。随着储存温度的升高,储存气氛对减少大肠杆菌 ATCC 25922 的存活种群和 D75 值的影响变得更加显着。在气调包装 (MAP) 下在 24°C 下储存 12 个月后,观察到存活种群减少 2.55 log CFU/g,D75 值减少 31.2%,并在 75°C 下保持 50.4 分钟至少可以达到大肠杆菌 ATCC 25922 减少 4 对数,这是美国农业部安全出口所要求的。在环境温度下在 MAP 下储存可能会抑制存活的种群并降低肠炎沙门氏菌 PT 30 在低水分杏仁粉中的耐热性。在气调包装 (MAP) 下在 24°C 下储存 12 个月后观察到 D75 值的 2%,并且在 75°C 下保持 50.4 分钟可以使大肠杆菌 ATCC 25922 至少减少 4-log,这美国农业部要求安全出口。在环境温度下在 MAP 下储存可能会抑制存活的种群并降低肠炎沙门氏菌 PT 30 在低水分杏仁粉中的耐热性。在气调包装 (MAP) 下在 24°C 下储存 12 个月后观察到 D75 值的 2%,并且在 75°C 下保持 50.4 分钟可以使大肠杆菌 ATCC 25922 至少减少 4-log,这美国农业部要求安全出口。在环境温度下在 MAP 下储存可能会抑制存活的种群并降低肠炎沙门氏菌 PT 30 在低水分杏仁粉中的耐热性。
更新日期:2018-04-01
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