Background and purpose of the study

The use of decellularised biological heart valves in the replacement of damaged heart valves offers a promising solution to reduce the degradation issues associated with existing cryopreserved allografts. The purpose of this study was to assess the effect of low concentration sodium dodecyl sulphate decellularisation on the in vitro biomechanical and hydrodynamic properties of cryopreserved human aortic and pulmonary roots.

Method

The biomechanical and hydrodynamic properties of cryopreserved decellularised human aortic and pulmonary roots were fully characterised and compared to cellular human aortic and pulmonary roots in an unpaired study. Following review of these results, a further study was performed to investigate the influence of a specific processing step during the decellularisation protocol (‘scraping’) in a paired comparison, and to improve the method of the closed valve competency test by incorporating a more physiological boundary condition.

Results

The majority of the biomechanical and hydrodynamic characteristics of the decellularised aortic and pulmonary roots were similar compared to their cellular counterparts. However, several differences were noted, particularly in the functional biomechanical parameters of the pulmonary roots. However, in the subsequent paired comparison of pulmonary roots with and without decellularisation, and when a more appropriate physiological test model was used, the functional biomechanical parameters for the decellularised pulmonary roots were similar to the cellular roots.

Conclusion

Overall, the results demonstrated that the decellularised roots would be a potential choice for clinical application in heart valve replacement.

中文翻译：

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去细胞人肺和主动脉根的体外生物力学和流体力学表征

研究背景和目的

使用脱细胞生物心脏瓣膜替代受损的心脏瓣膜提供了一种有希望的解决方案，以减少与现有冷冻保存的同种异体移植物相关的降解问题。这项研究的目的是评估低浓度十二烷基硫酸钠脱细胞对冷冻保存的人主动脉和肺根的体外生物力学和流体力学特性的影响。

方法

低温保存的脱细胞人主动脉和肺根的生物力学和流体力学特性已得到充分表征，并在一项未配对的研究中与细胞人主动脉和肺根进行了比较。在审查了这些结果之后，进行了进一步的研究，以成对比较的方式研究了脱细胞方案（“刮擦”）过程中特定加工步骤的影响，并通过结合更具生理意义的方法来改进闭阀能力测试的方法。边界条件。

结果

与去细胞主动脉和肺根相比，去细胞主动脉和肺根的大部分生物力学和流体力学特征均相似。但是，注意到一些差异，特别是在肺根的功能生物力学参数上。但是，在随后的有或没有脱细胞肺根的成对比较中，当使用更合适的生理测试模型时，用于脱细胞肺根的功能生物力学参数与细胞根相似。

结论

总体而言，结果表明脱细胞根将是临床应用在心脏瓣膜置换术中的潜在选择。