Cellular actions of N-(3-oxododecanoyl)-l-homoserine-lactone (ODHL), a quorum sensing molecule of bacteria, were studied on rat thymocytes using a flow cytometer with appropriate fluorescent dyes to elucidate the effects of ODHL on host cells. A bell-shaped concentration-response relation was observed in the ODHL-induced changes in cellular glutathione content ([GSH]i). ODHL concentration-dependently increased intracellular Zn²⁺ levels ([Zn²⁺]i) and cellular O₂⁻ content ([O₂⁻]i). The bell-shaped relation induced by ODHL can be explained as follows: a low concentration of ODHL is expected to induce moderate oxidative stress that intracellularly releases Zn²⁺ by converting thiols to disulfides. A slight elevation of [Zn²⁺]i may increase the [GSH]i. On the other hand, it is likely that a high concentration of ODHL causes severe oxidative stress that further causes both the decrease in [GSH]i and the increase in [Zn²⁺]i. Excessive increase in [Zn²⁺]i may augment oxidative stress that further decreases the [GSH]i. Other notable actions induced by ODHL included the elevation of [Zn²⁺]i by Zn²⁺ influx and the increase in [GSH]i under Zn²⁺-free conditions. Therefore, it is suggested that ODHL elicits diverse actions on host cells.

使用具有适当荧光染料的流式细胞仪研究大鼠群体感应分子N-（3-氧十二烷酰基）-1-高丝氨酸-内酯（ODHL）的细胞作用，以阐明ODHL对宿主细胞的影响。在ODHL诱导的细胞谷胱甘肽含量（[GSH] i）变化中观察到钟形的浓度-反应关系。ODHL浓度依赖性的细胞内增加的Zn ²⁺水平（[锌²⁺ ] i）和蜂窝ö ₂ ^-含量（〔O ₂ ^- ] i）的。ODHL引起的钟形关系可以解释如下：ODHL浓度低会诱导中等的氧化应激，从而在细胞内释放Zn ²⁺通过将硫醇转化为二硫化物。[Zn ²⁺ ] i的略微升高可能会增加[GSH] i。另一方面，高浓度的ODHL可能引起严重的氧化应激，这进一步导致[GSH] i降低和[Zn ²⁺ ] i升高。[Zn ²⁺ ] i的过度增加可能会增加氧化应激，从而进一步降低[GSH] i。通过ODHL诱导其他值得注意的行动包括的[Zn组成的高程²⁺ ] i增加的Zn ²⁺内流和Zn下增加[GSH] i的²⁺ -free条件。因此，建议ODHL在宿主细胞上引起多种作用。