Abstract Laser processing technique has been an effective method to enhance the medical implant’s biocompatibility through forming specific textures on titanium alloy implant surface. In this paper, a 1064 nm pulsed picosecond laser on TC4 titanium alloy implant samples was conducted to obtain optimal parameters for well-structured textures, controllable micro-grooves and improved osteoblast adhesion and proliferation. Picosecond laser technique is generally characterized by the high flexibility and advanced properties, which could make structural features including topography quality and detailed dimensions expediently improved and changed by adjusting laser processing parameters. To obtain the optimal laser processing parameters for desired microstructures, orthogonal experiments and related SEM, EDS and SPM analysis were conducted under various laser powers, repetition frequencies, scan speeds and scan numbers. Thus, the correlation between picosecond laser processing parameters and its corresponding microstructure feature of the micro-grooves was established. In addition, the immunofluorescence detection of cell actin cytoskeleton shows that structured textures can promote cell adhesion and play an important role in cell contact guidance.

中文翻译：

---

研究皮秒激光纹理化对钛合金微观结构和生物功能化的影响

摘要 激光加工技术是通过在钛合金种植体表面形成特定纹理来提高医用种植体生物相容性的有效方法。在本文中，对 TC4 钛合金植入物样品进行了 1064 nm 脉冲皮秒激光，以获得结构良好的纹理、可控的微凹槽和改善的成骨细胞粘附和增殖的最佳参数。皮秒激光技术通常具有高灵活性和先进性能的特点，可以通过调整激光加工参数方便地改善和改变包括形貌质量和细节尺寸在内的结构特征。为了获得所需微结构、正交实验和相关 SEM 的最佳激光加工参数，EDS 和 SPM 分析在不同的激光功率、重复频率、扫描速度和扫描次数下进行。因此，建立了皮秒激光加工参数与其相应的微凹槽微观结构特征之间的相关性。此外，细胞肌动蛋白细胞骨架的免疫荧光检测表明，结构化纹理可以促进细胞粘附并在细胞接触引导中发挥重要作用。