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Mechanistic insight in potential dual role of sinigrin against Helicoverpa armigera
Phytochemistry ( IF 3.8 ) Pub Date : 2018-01-01 , DOI: 10.1016/j.phytochem.2017.10.014
Aniruddha R. Agnihotri , Chaitanya V. Hulagabali , Anmol S. Adhav , Rakesh S. Joshi

The adverse effect of glucosinolates on diverse phytophagous insects is well documented, but its impact on insect physiology has remained enigmatic. Here we report insights into detrimental effects of plant glucosinolate molecule, sinigrin, on Helicoverpa armigera growth and development. In-silico screening of multiple glucosinolates predicted sinigrin as one of the potential inhibitor of H. armigera cathepsin B and L. Insects fed on sinigrin containing diet showed significantly reduced growth (20-30%), delayed pupation (10-15%), decreased fecundity (50-80%) and developmental abnormalities. Further, sinigrin showed 50-60% inhibition of ex-vivo cathepsin like activity which might be a reason for growth and development related abnormalities. In-vitro and mass spectrometry studies highlighted the cytotoxicity caused due to the hydrolysis of sinigrin, into toxic isothiocyanates, in presence of H. armigera whole body extract. In conclusion, insect cathepsin inhibition and isothiocyanate mediated cytotoxicity lead to the dual adverse effect of sinigrin on H. armigera.

中文翻译:

辛尼格林对棉铃虫潜在双重作用的机制洞察

硫代葡萄糖苷对多种植食性昆虫的不利影响已得到充分证明,但其对昆虫生理学的影响仍然是个谜。在这里,我们报告了对植物芥子油苷分子 sinigrin 对棉铃虫生长和发育的不利影响的见解。多种硫代葡萄糖苷的计算机内筛选预测红豆杉是棉铃虫组织蛋白酶 B 和 L 的潜在抑制剂之一。以含有红豆杉的饮食喂养的昆虫显示生长显着降低 (20-30%),延迟化蛹 (10-15%),生育力下降 (50-80%) 和发育异常。此外,sinigrin 表现出对离体组织蛋白酶样活性的 50-60% 抑制,这可能是生长和发育相关异常的原因。体外和质谱研究强调了由红霉素水解引起的细胞毒性,在棉铃虫全身提取物的存在下,转化为有毒的异硫氰酸盐。总之,昆虫组织蛋白酶抑制和异硫氰酸酯介导的细胞毒性导致了红毛虫对棉铃虫的双重不利影响。
更新日期:2018-01-01
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