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Investigation of the chemical mechanisms involved in the electropulsation of membranes at the molecular level
Bioelectrochemistry ( IF 5 ) Pub Date : 2017-09-11 , DOI: 10.1016/j.bioelechem.2017.09.005
Marie Breton , Lluis M. Mir

The chemical consequences of electropulsation on giant unilamellar vesicles (GUVs), in particular the possible oxidation of unsaturated phospholipids, have been investigated by mass spectrometry, flow cytometry and absorbance methods. Pulse application induced oxidation of the GUV phospholipids and the oxidation level depended on the duration of the pulse. Light and O2 increased the level of pulse-induced lipid peroxidation whereas the presence of antioxidants either in the membrane or in the solution completely suppressed peroxidation. Importantly, pulse application did not create additional reactive oxygen species (ROS) in GUV-free solution. Lipid peroxidation seems to result from a facilitation of the lipid peroxidation by the ROS already present in the solution before pulsing, not from a direct pulse-induced peroxidation. The pulse would facilitate the entrance of ROS in the core of the membrane, allowing the contact between ROS and lipid chains and provoking the oxidation. Our findings demonstrate that the application of electric pulses on cells could induce the oxidation of the membrane phospholipids since cell membranes contain unsaturated lipids. The chemical consequences of electropulsation will therefore have to be taken into account in future biomedical applications of electropulsation since oxidized phospholipids play a key role in many signaling pathways and diseases.



中文翻译:

在分子水平上研究膜电脉冲涉及的化学机理

已经通过质谱,流式细胞术和吸收法研究了电脉冲对巨大单层囊泡(GUV)的化学作用,特别是不饱和磷脂可能的氧化作用。脉冲施加引起了GUV磷脂的氧化,氧化水平取决于脉冲的持续时间。光和O 2增加了脉冲诱导的脂质过氧化的水平,而膜中或溶液中抗氧化剂的存在则完全抑制了过氧化。重要的是,脉冲施加不会在不含GUV的溶液中产生额外的活性氧(ROS)。脂质过氧化作用似乎是由脉冲前溶液中已经存在的ROS促进脂质过氧化作用所致,而不是直接脉冲诱导的过氧化作用所致。脉冲将促进ROS进入膜的核心,从而允许ROS与脂质链之间的接触并引起氧化。我们的发现表明,在细胞上施加电脉冲可能会诱导膜磷脂的氧化,因为细胞膜含有不饱和脂质。

更新日期:2017-09-11
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