Acute limb ischaemia is caused by compromised tissue perfusion and requires immediate attention to reduce the occurrence of secondary complications that could lead to amputation or death. To address this, we have developed a novel platinum (Pt)-based electrochemical oxygen (O₂) device for future applications in clinical monitoring of peripheral tissue ischaemia. The effect of integrating a Pt pseudo-reference electrode into the O₂ device was investigated in vitro with an optimum reduction potential of − 0.80 V. A non-significant (p = 0.11) decrease in sensitivity was recorded when compared against an established Pt-based O₂ sensor operating at − 0.65 V. Furthermore, a biocompatible clinical sensor (ClinOX) was designed, demonstrating excellent linearity (R² = 0.99) and sensitivity (1.41 ± 0.02 nA μM^− 1) for O₂ detection. Significant rapid decreases in the O₂ current during in vivo ischaemic insults in rodent limbs were reported for Pt-Pt (p < 0.001) and ClinOX (p < 0.01) and for ClinOX (p < 0.001) in porcine limbs. Ex vivo sensocompatibility investigations identified no significant difference (p = 0.08) in sensitivity values over 14 days of exposure to tissue homogenate. The Pt-Pt based O₂ design demonstrated high sensitivity for tissue ischaemia detection and thus warrants future clinical investigation.

急性肢体缺血是由组织灌注不足引起的，需要立即注意以减少可能导致截肢或死亡的继发并发症的发生。为了解决这个问题，我们开发了一种新型的基于铂（Pt）的电化学氧（O ₂）装置，用于将来在周围组织局部缺血的临床监测中的应用。的Pt伪参比电极集成到将O的效果₂装置进行了研究在体外用的最佳还原电位- 0.80 V.非显著（p  = 0.11），当针对已建立的铂-相比，灵敏度下降，记录基于O ₂传感器在操作- 0.65 V.此外，生物相容的临床传感器（ClinOX）的设计，这表明优异的线性度（R ²  = 0.99）和灵敏度（1.41±0.02 nA的μM ^{- 1}）代表O ₂的检测。据报道， 在猪肢体中，Pt-Pt（p  <0.001）和ClinOX（p  <0.01）和ClinOX（p <0.001）在啮齿动物肢体的体内缺血损伤期间O ₂电流显着降低。离体的感觉相容性研究发现， 在暴露于组织匀浆的14天中，敏感性值无显着差异（p = 0.08）。基于Pt-Pt的O_2个设计显示出对组织局部缺血检测的高灵敏度，因此值得将来进行临床研究。