This study evaluated the effect of temperature on methane production by CO₂ reduction during microbial electrosynthesis (MES) with a mixed-culture biocathode. Reactor performance, in terms of the amount and rate of methane production, current density, and coulombic efficiency, was compared at different temperatures. The microbial properties of the biocathode at each temperature were also analyzed by 16S rRNA gene sequencing. The results showed that the optimum temperature for methane production from CO₂ reduction in MES with a mixed-culture cathode was 50 °C, with the highest amount and rate of methane production of 2.06 ± 0.13 mmol and 0.094 ± 0.01 mmol h^− 1, respectively. In the mixed-culture biocathode MES, the coulombic efficiency of methane formation was within a range of 19.15 ± 2.31% to 73.94 ± 2.18% due to by-product formation at the cathode, including volatile fatty acids and hydrogen. Microbial analysis demonstrated that temperature had an impact on the diversity of microbial communities in the biofilm that formed on the MES cathode. Specifically, the hydrogenotrophic methanogen Methanobacterium became the predominant archaea for methane production from CO₂ reduction, while the abundance of the aceticlastic methanogen Methanosaeta decreased with increased temperature.

这项研究评估了温度对混合培养生物阴极微生物电合成（MES）过程中CO ₂还原产生甲烷的影响。在不同温度下，比较了反应器性能，包括甲烷生成量和速率，电流密度和库伦效率。还通过16S rRNA基因测序分析了每个温度下生物阴极的微生物特性。结果表明，在混合培养阴极条件下，MES还原CO ₂产生甲烷的最佳温度为50°C，最高甲烷产生量和速率为2.06±0.13 mmol和0.094±0.01 mmol h ^-1， 分别。在混合培养生物阴极MES中，由于在阴极处形成副产物，包括挥发性脂肪酸和氢，甲烷形成的库伦效率在19.15±2.31％至73.94±2.18％的范围内。微生物分析表明，温度对在MES阴极上形成的生物膜中微生物群落的多样性有影响。具体而言，氢营养型产甲烷菌甲烷甲烷菌是从CO ₂还原产生甲烷的主要古细菌，而随着温度的升高，回弹型产甲烷菌甲烷甲烷菌的丰度降低。