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Epitope‐Resolved Detection of Peanut‐Specific IgE Antibodies by Surface Plasmon Resonance Imaging
ChemBioChem ( IF 3.2 ) Pub Date : 2018-01-04 , DOI: 10.1002/cbic.201700513
Min Shen 1 , Amit A. Joshi 1 , Raghu Vannam 1 , Chandra K. Dixit 1 , Robert G. Hamilton 2 , Challa V. Kumar 1, 3 , James F. Rusling 1, 4 , Mark W. Peczuh 1
Affiliation  

Predicting peanut allergies: For the first time, epitope‐resolved detection of peanut‐specific IgE antibodies was achieved on a surface plasmon resonance imaging (SPRi) immunoarray using superparamagnetic bead‐antibody conjugates (MB‐Ab2). The level of IgE antibodies specific for a prominent peptide epitope, Ara h2–3, showed promise for predicting peanut sensitization.
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中文翻译:

通过表面等离振子共振成像进行表位解析的花生特异性IgE抗体检测

预测花生过敏:首次使用超顺磁珠-抗体结合物(MB-Ab 2)在表面等离子体共振成像(SPRi)免疫阵列上实现了表位分辨的花生特异性IgE抗体检测。对突出的肽表位,Ara h2-3特异性的IgE抗体的水平显示了预测花生致敏性的希望。
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更新日期:2018-01-04
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