A system based on batch injection analysis (BIA) associated with amperometric detection at screen‐printed carbon electrode was used for the precise and rapid quantification of the anesthetics compounds benzocaine and tricaine in fresh fish fillets. Along this study, the best conditions for the BIA‐amperometry system were stablished for the rapid determination of these compounds. The results obtained demonstrate that the proposed method is an interesting alternative to the chromatographic methods, once it allows to perform rapid analysis (more than 300 injections per hour) with low limits of detection (3.02×10⁻⁸ mol L⁻¹ for benzocaine and 3.19×10⁻⁸ mol L⁻¹ for tricaine), using just 80 μL of sample for each analysis. Furthermore, it was possible to obtain high repeatability for both compounds analyzed, demonstrating good performance. The simple sample preparation developed in this study drastically reduced the amount of fat in the fish extract, favoring precision, as shown by the results of the recovery studies of both anesthetics contained in the fish samples (values above 99 % for both analytes).

中文翻译：

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BIA电流检测法测定鱼片中的苯佐卡因和三卡因

基于批注入分析（BIA）的系统与丝网印刷碳电极上的安培检测相关的系统用于精确快速定量新鲜鱼片中的麻醉剂化合物苯佐卡因和三卡因。在这项研究中，为快速测定这些化合物建立了BIA安培法系统的最佳条件。获得的结果表明，所提出的方法可以快速进行分析（每小时进样300次以上），并且检出限较低（苯并卡因和苯并卡因的检出限为3.02×10 ^-8  mol L ^-1），因此它是色谱法的有趣替代方法。3.19×10 ^-8 摩尔L ^-1对于三卡因），每次分析仅使用80μL样品。此外，对于所分析的两种化合物均可以获得较高的重复性，这表明其具有良好的性能。如鱼样品中所含两种麻醉剂的回收率研究结果（两种分析物的值均高于99％）所示，本研究开发的简单样品制备方法可大幅减少鱼提取物中的脂肪含量，从而提高了精确度。