An enzyme‐based XOR logic gate was realized at interface of an alginate‐modified electrode. The biocatalytic production of H₂O₂ inside the alginate film was controlled by logically processed input signals. The in situ generated H₂O₂ was decomposed to yield free radicals in a Fenton‐type reaction catalyzed by iron cations, which were present in the alginate film as cross‐linkers stabilizing the hydrogel. The produced free radicals (•OH, •OOH) resulted in decomposition/dissolution of the alginate film removing it from the electrode surface and stimulating release process of magnetic nanoparticles (MNPs) functionalized with a fluorescent dye and entrapped in the alginate film. The release of the MNPs was analyzed by following fluorescence appearing in the solution. The release process followed the logic features of the XOR gate. The present system is the first realization of the enzyme‐based XOR gate functionally integrated with the downstream actuation process in the form of the signal‐stimulated release.

中文翻译：

---

基于酶的XOR Logic Gate激活的纳米物种释放系统

在藻酸盐修饰电极的界面处实现了基于酶的XOR逻辑门。通过逻辑处理的输入信号控制藻酸盐膜内部H ₂ O ₂的生物催化产生。该原位生成的ħ ₂ ö ₂被铁阳离子催化的Fenton型反应中分解出自由基，这些阳离子作为稳定水凝胶的交联剂存在于藻酸盐膜中。产生的自由基（•OH，•OOH）导致藻酸盐膜的分解/溶解，将其从电极表面去除，并刺激了用荧光染料官能化并截留在藻酸盐膜中的磁性纳米颗粒（MNP）的释放过程。通过追踪溶液中出现的荧光来分析MNP的释放。释放过程遵循XOR门的逻辑功能。本系统是基于酶的异或门的首个实现，该异或门功能性地以信号刺激释放的形式与下游驱动过程集成在一起。