The biodiesel fuel ethyl oleate (fatty acid ethyl ester, FAEE) was produced using Novozym^® 435 (immobilized lipase) by transesterification of a mixture of ethanol and triolein in a fixed-bed reactor operated in circulating batch mode. The miscibility of the mixtures during transesterification was predicted thermodynamically and the conversion ratio of triolein was studied as a function of reaction time. The yield of ethyl oleate increased when the feed molar composition of ethanol was increased. The glycerin by-product could be removed from the enzyme particles by the circulating feed mixture. The inactivation of Novozym^® 435 by glycerin was also examined in the circulating batch mode experiments, and an effective reaction model was proposed to describe the inactivation of Novozym^® 435 and ethanol effect during the transesterification reaction by using the modified Michaelis-Menten equation.

生物柴油燃料油酸乙酯（脂肪酸乙酯，FAEE）使用的Novozym产生^® 435（固定化脂肪酶）的乙醇的混合物中的酯交换和在一个固定床反应器中的三油酸甘油酯在循环间歇方式操作。热力学上预测了酯交换过程中混合物的溶混性，并研究了三油精的转化率与反应时间的关系。当乙醇的进料摩尔组成增加时，油酸乙酯的产率增加。甘油副产物可以通过循环的进料混合物从酶颗粒中除去。的Novozym的失活^®由甘油435还检测在循环间歇方式的实验中，并提出了一种有效的反应模型来描述的Novozym的失活^® 435和通过使用修改后的米氏方程的酯交换反应过程中乙醇的影响。